小剂量X线对小鼠细胞免疫功能影响  

Effect of low dose X-ray irradiation on cell immuno-function of BALB/C mouse

作　　者：王存邦[1] 白海[1] 葸瑞[1] 徐淑芬[1] 张茜[1] 周进茂[1] 吴涛[1] 谵台林芳 

机构地区：[1]兰州军区兰州总医院全军血液病中心,兰州730050

出　　处：《中国公共卫生》2010年第5期655-656,共2页Chinese Journal of Public Health

基　　金：全军"十一五"杰出人才基金(06J005);兰州军区医药科研基金(LXH-2007006)

摘　　要：目的动态观察小剂量X线照射后BALB/C小鼠的细胞免疫功能变化情况。方法75只小鼠分成3组,经2和3 Gy X线照射后,应用流式细胞仪每周测定外周血T细胞亚群及NK细胞,观察5周。结果2Gy照射后1周,小鼠外周血CD3+、CD4+、CD8+、CD1+6CD5+6细胞降至24.76±1.87,22.87±1.71,1.07±0.07,4.64±0.78(P<0.05),并呈进行性降低,至第3周开始恢复,第4周恢复正常;3Gy照射后1周,小鼠外周血CD3+、CD4+、CD8+、CD1+6CD5+6细胞分别降至4.11±0.21,2.33±0.91,0.17±0.07,2.33±0.18(P<0.01),并呈进行性降低,至第4周开始恢复,第5周恢复正常;在同一时间,3Gy照射较2Gy照射所导致的细胞亚群降低的程度更为明显(P<0.05)。结论BALB/C小鼠经小剂量X线照射后细胞免疫功能下降明显,4～5周后可恢复正常。Objective To explore the effect of low dose X-ray irradiation on cell immuno-function of BALB/C mouse.Methods The mice were divided into three groups and exposed to 0,2,and 3Gy X-ray,respectively.Then T cell subsets and natural killer（NK） cell were examinde with FACS every week for five weeks.Results One week after 2Gy irradiation,the CD＋3,CD＋4,CD＋8,CD＋16CD＋56 cells in peripheral blood were 24.76±1.87,22.87±1.71,1.07±0.07,4.64±0.78（P0.05） with progressive decrease and recovered in the fourth week.One week after 3 Gy irradiation,the CD＋3,CD＋4,CD＋8,CD＋16 CD＋56 cells in peripheral blood were 4.11±0.21,2.33±0.91,0.17±0.07,2.33±0.18（P0.01） with progressive decrease and recovered in the fifth week.The decrease of subsets cell was more obvious in mice irradiated with 3Gy than that of the mice with 2Gy irridiation（P0.05）.Conclusion Cell immuno-function of BALB/C mouse declined obviously after low dose X-ray irradiation and recovered gradually after 4-5 weeks.

关 键 词：BALB/C小鼠 小剂量X线 照射 T细胞亚群 NK细胞 

分 类 号：R57[医药卫生—消化系统]