Confirmation of combinational effects of calcium with other metals in a paper recycling mill effluent on nematode lifespan with toxicity identification evaluation method  

作　　者：Dayong Wang,Yang Wang,Lulu Shen Key Laboratory of Developmental Genes and Human Disease in Ministry of Education,Department of Genetics and Developmental Biology,Southeast University Medical School,Nanjing 210009,China. 

出　　处：《Journal of Environmental Sciences》2010年第5期731-737,共7页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 30771113,30870810);the Program for New Century Excellent Talents in University;provided by the Caenorhabditis Genetics Center (Funded by the NIH,National Center for Foundation from Research Resource,USA)

摘　　要：We used toxicity identification evaluation （TIE） method to confirm the combinational effects of identified toxic metals in a paper recycling mill effluent in inducing the decreased lifespan in nematode Caenorhabditis elegans.Exposure to Ca ＋ Al caused more severely decreased lifespan than that exposed to Ca,or Al;and exposure to Ca ＋ Fe induced more severely decreased lifespan than that exposed to Ca,or Fe.Exposure to Ca＋Al＋Fe caused more severely decreased lifespan than that exposed to Ca,or Ca＋Fe.Moreover,the baseline toxicity on lifespan was doubled by doubling the concentration of combined metals （Ca＋Al＋Fe） in spiking test in original effluent （oe）,and lifespan defects in oe＋Ca＋Al＋Fe exposed nematodes were more severe than that in Ca＋Al＋Fe exposed nematode.Therefore,Ca＋Al＋Fe exposure may largely explain the formation of decreased lifespan induced by the examined industrial effluent.Furthermore,the observed reduction of lifespan induced by the combination of high level of Ca with other metals may be at least partially independent of the insulin-like pathway.We used toxicity identification evaluation （TIE） method to confirm the combinational effects of identified toxic metals in a paper recycling mill effluent in inducing the decreased lifespan in nematode Caenorhabditis elegans.Exposure to Ca ＋ Al caused more severely decreased lifespan than that exposed to Ca,or Al;and exposure to Ca ＋ Fe induced more severely decreased lifespan than that exposed to Ca,or Fe.Exposure to Ca＋Al＋Fe caused more severely decreased lifespan than that exposed to Ca,or Ca＋Fe.Moreover,the baseline toxicity on lifespan was doubled by doubling the concentration of combined metals （Ca＋Al＋Fe） in spiking test in original effluent （oe）,and lifespan defects in oe＋Ca＋Al＋Fe exposed nematodes were more severe than that in Ca＋Al＋Fe exposed nematode.Therefore,Ca＋Al＋Fe exposure may largely explain the formation of decreased lifespan induced by the examined industrial effluent.Furthermore,the observed reduction of lifespan induced by the combination of high level of Ca with other metals may be at least partially independent of the insulin-like pathway.

关 键 词：LIFESPAN CALCIUM combinational toxicity toxicity identification evaluation （TIE） Caenorhabditis elegans 

分 类 号：X703[环境科学与工程—环境工程]