癌组织中miRNA表达谱的变化与NSCLC放疗敏感性的关系  

Change of miRNA expression profile in non-small cell lung cancer tissue and the correlation between it and radiotherapy sensitive

作　　者：崔爽爽[1,2] 杜利清[1,2] 王月英[1,2] 孟爱民[1,2] 王小春[1,2] 赵永成[1,2] 

机构地区：[1]北京协和医学院,北京100730 [2]中国医学科学院放射医学研究所

出　　处：《山东医药》2010年第18期12-13,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30901723);教育部博士点新教师基金资助项目(200800231052);北京协和医学院CMB基金资助项目(A2009002);放射医学研究所人才基金(SR0825);探索基金(ST0834)资助项目

摘　　要：目的观察非小细胞肺癌(NSCLC)患者癌组织中微型核糖核酸(miRNA)表达谱的变化,并探讨其与NSCLC放疗敏感性的关系。方法将30例NSCLC患者根据总体生存率和远端转移情况分为放疗敏感组和不敏感组,采用miRNA表达谱芯片检测癌组织中的miRNA表达谱。结果与放疗不敏感组相比,放疗敏感组中有5个miRNA(miR-126、miR-let-7a、miR-495、miR-451、miR-128b)表达显著上调(P均<0.01),7个miRNA(miRNA-130a、miRNA-106b、miRNA-19b、miRNA-22、miRNA-15b、miRNA-17-5p、miRNA-21)表达显著下调(P均<0.01)。结论放疗敏感和不敏感NSCLC患者癌组织miRNA表达谱有差异,这种差异可能与NSCLC的放疗敏感性有关。Objective To observe the change of miRNA expression profile in non-small cell lung cancer（NSCLC）tissue,and explore the correlation between it and radiotherapy sensitive.Methods 30 NSCLC patients were divided into radiotherapy sensitive group and resistant group.Expression profile of miRNA was detected by microarray assay and the results were validated by quantitative RT-PCR.Results Comparing with resistant patients,5 miRNA（miR-126,miR-let-7a,miR-495,miR-451,miR-128b）were significantly up-regulated（P0.01） and 7 miRNA（miRNA-130a,miRNA-106b,miRNA-19b,miRNA-22,miRNA-15b,miRNA-17-5p,miRNA-21）were greatly down-regulated（P0.01） in radiotherapy sensitive group.Conclusion There was difference in radiotherapy sensitive and resistant NSCLC group.It may be correlated with the radiotherapy sensitivity of non-small cell lung cancer.

关 键 词：肺肿瘤 非小细胞肺癌 微型核糖核酸 放射疗法 

分 类 号：R734.2[医药卫生—肿瘤]