微生物絮凝剂产生菌的筛选及培养条件的优化  

Screening and condition optimization for the microbial flocculant-producing bacteria

作　　者：张晓杰[1] 季丽媛[2] 

机构地区：[1]吉林化工学院环境与生物工程学院,吉林吉林132022 [2]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《吉林化工学院学报》2010年第2期17-20,共4页Journal of Jilin Institute of Chemical Technology

摘　　要：对絮凝剂产生菌MF1进行了培养基成分的优化和培养条件的正交实验研究.实验表明:菌MF1产絮凝剂的最佳培养基成分为葡萄糖20 g;尿素0.5 g;K2HPO45.0 g;MgSO4.7H2O 0.2 g;NaCl 0.1 g;酵母粉0.5 g;H2O 1 000 mL;培养基初始pH值为6.0、培养温度为32℃、摇床转速为120 r/min.在上述最适培养条件下,培养48 h产生的絮凝剂对高岭土悬浮液的絮凝率可达85.88%.This paper carried out the orthogonal experiment about microbial flocculant-producing bacteria MF1.The cultivation conditions were optimized.The optimum fermentation medium are determined as follows：glucose 20 g;urea 0.5 g;K2HPO4 5.0 g;MgSO4·7H2O 0.2 g;NaCl 0.1 g;yeast powder 0.5 g;H2O 1000 mL;initial pH value is 6.0;cultivation temperature to 32 ℃;the rotational speed of oscillator is at 120 rpm;cultivation time is 48 h.Under these conditions,the flocculating efficiency of flocculant for kaolin is 85.88%.

关 键 词：微生物絮凝剂 菌种筛选 正交实验 培养条件优化 

分 类 号：X703.5[环境科学与工程—环境工程]