病理性瘢痕和正常皮肤来源成纤维细胞CD90、α-SMA表达的研究  

CD90 and α-SMA Expression and Their Correlation of Fibroblasts from Abnormal Scars and Normal Skin

作　　者：苏卫国[1] 王平丽[2] 李永林[3] 李冰燕[4] 牛希华[1] 娄季鹤[1] 戴建新[2] 

机构地区：[1]郑州市第一人民医院烧伤科,河南郑州450004 [2]河南职工医学院,河南郑州450003 [3]苏州大学附属第一医院烧伤整形科,江苏苏州215006 [4]苏州大学放射医学与公共卫生学院,江苏苏州215007

出　　处：《河南职工医学院学报》2010年第2期113-115,F0003,共4页Journal of Henan Medical College For Staff and Workers

摘　　要：目的检测体外培养的病理性瘢痕和正常皮肤成纤维细胞中CD90、α-SMA表达情况,并分析二者的相关性。方法取手术切除的瘢痕疙瘩4例、增生性瘢痕12例、正常皮肤(瘢痕边缘)16例,每组标本中选取特异性较强的组织块各3例,原代培养不同组织来源的成纤维细胞,取第3代细胞制成细胞爬片,免疫荧光双重标记(CD90和α-SMA)细胞,通过计算每系成纤维细胞CD90、α-SMA的荧光值,检测成纤维细胞中CD90、α-SMA的表达情况,并分析CD90和α-SMA表达的关系。结果增生性瘢痕来源成纤维细胞的CD90表达强于正常皮肤的成纤维细胞(P<0.05),瘢痕疙瘩来源成纤维细胞的CD90表达和正常皮肤的表达无差异(P>0.05);增生性瘢痕与其周围皮肤、瘢痕疙瘩与其周围正常皮肤来源成纤维细胞的α-SMA表达均无差异(P>0.05);在同一细胞内CD90和α-SMA具有共区域和共趋势表达的特点。结论增生性瘢痕来源的成纤维细胞存在CD90多量表达的特异表型,瘢痕成纤维细胞CD90和α-SMA有共区域表达的特点和一致的表达趋势,二者可能有一定的协同关系。Objective To investigate the differences among CD90 and α-SMA expression,and their correlation of fibroblasts from abnormal scars and normal skin in vitro.Methods The 16 abnormal scar tissues and matched normal skin were divided into keloid group（n=4）,hypertrophic scar group（n=12）,normal skinkeloid group（n=4） and normal skin-hypertrophic scar group（n=12）.Fibroblasts were isolated from the three specific samples of every group by primary culture.After three passages,CD90 and α-SMA expression in fibroblasts grown on cover glass were detected through calculating the fluorescent quantitation.Results CD90 expression in hypertrophic scar-derived fibroblasts was significantly more than that in normal skin-derived fibroblasts（P0.05）.There was no difference in CD90 expression between the fibroblasts derived from keloid and normal skin（P0.05）.CD90 and α-SMA expression were co-location and had the coherent tendency in single fibroblast.Conclusion There are some fibroblasts derived from hypertrophic scars significantly expressing CD90 in vitro.CD90 expression and α-SMA are co-location and coherent in single fibroblast and they may have some synergistic relationships.

关 键 词：瘢痕疙瘩 增生性瘢痕 成纤维细胞 CD90 

分 类 号：R361.2[医药卫生—病理学]