RP-HPLC法同时测定坎离砂中阿魏酸和川芎嗪的含量  

Simultaneous Determination of Ferulic Acid and Chuanxiongzine in Kanlisha by RP-HPLC

作　　者：郭允 张琨[2] 赵旻[3] 洪博[3] 赵春杰[3] 

机构地区：[1]郑州市卫生学校,河南郑州450005 [2]河南职工医学院,河南郑州451191 [3]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《河南职工医学院学报》2010年第2期115-118,共4页Journal of Henan Medical College For Staff and Workers

摘　　要：目的建立坎离砂中阿魏酸（FA）和川芎嗪（CXZ）的同时含量测定方法。方法采用RP-HPLC法同时测定FA和CXZ的含量。色谱柱：D iamonsil（钻石）C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈-0.4%冰醋酸水溶液（三乙胺调pH 3.5）15∶85为流动相,流速为1.0 m l.m in-1,检测波长280 nm,进样量20μl。结果 FA、CXZ分别在14.35-328 mg.L-1（r=0.999 5）、6.91-158 mg.L-1（r=0.999 5）范围内呈良好的线性范围,平均回收率均大于99.0%,RSD小于1.5%（n=9）。结论该方法快速、准确、简便、专属性强、重现性好,可用于坎离砂的质量控制。目的建立坎离砂中阿魏酸（FA）和川芎嗪（CXZ）的同时含量测定方法。方法采用RP-HPLC法同时测定FA和CXZ的含量。色谱柱：Diamonsil（钻石）C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈-0.4%冰醋酸水溶液（三乙胺调pH3.5）15∶85为流动相,流速为1.0 ml.min-1,检测波长280 nm,进样量20μl。结果 FA、CXZ分别在14.35-328 mg.L-1（r=0.999 5）、6.91-158 mg.L-1（r=0.999 5）范围内呈良好的线性范围,平均回收率均大于99.0%,RSD小于1.5%（n=9）。结论该方法快速、准确、简便、专属性强、重现性好,可用于坎离砂的质量控制。Objective To establish method for the determination of Ferulic acid（FA） and Chuanxiongzine（CXZ） in kanlisha.Methods The determination of FA and CXZ was performed by RP-HPLC.They were separated on a Diamonsil C18 column（250 mm×4.6 mm,5 μm） using solvents of acetonitrile and 0.4% acetic acid aqueous（to adjust pH3.5 with triethylamine）（15∶85） as mobile phase.They were detected at 280 nm with flow rate as 1.0 mL·min-1,the column temperature was 25℃ and sample size was 20 μL.Results The calibration curve was linear in the range of 14.35~328 mg·L-1 for FA（r=0.999 5）,6.91~158 mg·L-1 for CXZ （r=0.999 5）.The average recoveries of samples were all over 99.0% and RSD was below 1.5%（n=9）.ConclusionThe method of content determination is rapid,accurate,specific and reproducible,and is suitable for the quality control of kanlisha.

关 键 词：坎离砂 当归 川芎 高效液相色谱 

分 类 号：R914.1[医药卫生—药物化学]