SD大鼠C6脑胶质瘤伽玛刀干预前后VEGF的表达变化  

Change of VEGF expression of SD rat C6 brain glioma model after gamma knife radiosurgery

作　　者：王鹏[1,2,3] 程勋全[4] 韩宏勋 于金明[2,3] 朱玉存[1] 马绍峰[1] 朱世友[1] 乔飞飞[1] 

机构地区：[1]合肥仁济肿瘤医院伽玛刀中心,安徽合肥230022 [2]山东省肿瘤防治院 [3]山东省肿瘤医院放射治疗科,山东济南250117 [4]安徽省立医院放射治疗科

出　　处：《中国实验诊断学》2010年第5期641-643,共3页Chinese Journal of Laboratory Diagnosis

基　　金：卫生部课题(WKJ2005-3-006)

摘　　要：目的通过对C6大鼠脑胶质瘤的伽玛刀治疗,检测伽玛刀治疗前后VEGF的变化,探讨伽玛刀治疗胶质瘤作用机制。方法 50只SD大鼠,采用立体定向法对40只SD大鼠进行动物造模,10只作为正常对照组,14 d后对其中20只进行伽玛刀治疗作为治疗组,20只作为肿瘤对照组。伽玛刀治疗后7 d,对所有大鼠处死并应用免疫组化法检测VEGF并进行统计学分析。结果 VEGF在正常大鼠基本没有表达,在肿瘤对照组大鼠中表达增强,伽玛刀照射组VEGF的表达降低。结论伽玛射线可降低大鼠C6脑胶质瘤VEGF表达。Objective To investigate the mechanism of gamma knife radiosurgery treatment of C6 glioma by examing the expression changes of VEGF.Methods 40 SD C6 brain glioma model was established by stereotactic implatation and 10 as normal control group.After 14 days,20 SD rats were given gamma knife radiosurgery and 20 SD rats as control group.7 days after gamma knife radiosurgery,all the rats were beheaded and given VEGF immunol histochemistry stains,and was given statistical analysis.Results There is little expression of VEGF in normal brain and intense expression in gliomas.After gamma knife radiosurgery the VEGF expression reduced significantly.Conclusion VEGF expression in glioma can be reduced by Gamma knife radiosurgery.

关 键 词：C6脑胶质瘤 伽玛刀 血管内皮生长因子 

分 类 号：R739.41[医药卫生—肿瘤]