GRIM-19的表达对人子宫颈癌细胞移植瘤细胞侵袭、凋亡的影响研究  

Study of effects of expression of GRIM-19 on invasion and apoptosis of cervical cancer cell xenograft tumor

作　　者：程勇[1] 周颖[2] 卫莹[2] 李敏[2] 冯定庆[2] 肖卫华[3] 凌斌[2] 

机构地区：[1]安徽医科大学附属省立医院肿瘤放疗科,安徽合肥230001 [2]安徽省分子医学重点实验室,安徽省立医院妇产科分子实验室 [3]中国科技大学生命科学院免疫学研究所

出　　处：《中国实验诊断学》2010年第5期733-735,共3页Chinese Journal of Laboratory Diagnosis

基　　金：安徽省自然科学基金项目(090413117);安徽省高等学校省级自然科学研究项目(KJ2010B375)

摘　　要：目的通过裸鼠体内试验探讨GRIM-19对子宫颈癌细胞株HeLa的侵袭、凋亡的影响。方法将HeLa/Con-trol、HeLa/GRIM-19接种到裸鼠皮下的移植瘤剥离,采用western Blot检测MMP-2、MMP-9基因的mRNA和蛋白水平表达情况;利用Tumel实验检测细胞凋亡。结果移植瘤的蛋白检测发现MMP-2、MMP-9基因的表达均下降,HeLa/GRIM-19移植瘤中凋亡细胞增多。结论 GRIM-19的表达可以抑制子宫颈癌细胞的侵袭、促进细胞凋亡,可能是子宫颈癌治疗的新靶点。Objective To explore the effects of expression of GRIM-19 on the invasion and apoptosis of cervical cancer in vivo.Methods Tumor tissue of the null mice was stripped after injection HeLa/Control、HeLa/GRIM-19 into the skin for 7 weeks.The protein level of gene expression was investigated by western Blot.Cell apoptosis was examined by Tunel assay.Results The growth of HeLa/GRIM-19 implanted tumor slowed down significantly（P0.01）.The protein expression of MMP-2 and MMP-9 was decreased in tumors bearing HeLa/GRIM-19.Apoptosis cells were increased in HeLa/GRIM-19 implanted tumor.Conclusion The expression of GRIM-19 can slow down the invasion of cervical cancer cells,and contribute to cell apoptosis.Suggesting that GRIM-19 may be a new target for the treatment of cervical cancer.

关 键 词：GRIM-19 转染 子宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]