牛分支杆菌DNA疫苗工程菌的高密度培养研究  

Study on the High Density Cultivation of Mycobacterium bovis DNA Vaccine Engineering Bacteria

作　　者：宋振忠[1,2] 徐志光[1] 黄炯[1,3] 薛英[1,3] 王延[1,3] 许礼义[1,2] 张读朴 鲁立柱 蔡宏[4] 呼西旦[1] 

机构地区：[1]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000 [2]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [3]新疆天康畜牧生物技术股份有限公司,新疆乌鲁木齐832023 [4]北京大学生命科学学院,北京100871

出　　处：《动物医学进展》2010年第5期9-13,共5页Progress In Veterinary Medicine

基　　金：新疆维吾尔自治区高技术研究发展计划项目(200811111)

摘　　要：对牛分支杆菌三价DNA疫苗工程菌进行了高密度培养的探索。通过摇瓶培养试验对3种培养基进行了筛选,选择出具有高密度培养潜力的半合成培养基作为中试规模的发酵罐培养基;经对培养温度、溶氧、pH、转速、补料方式等各种条件优化,培养的工程菌密度OD600达到22.92,细菌湿重达到27.173 g/L,质粒产量为1.44 mg/g湿菌。试验还对培养物质粒的稳定性进行了证实。Engineering bacteria with high density cultivation in Mycobacterium bovis DNA Vaccine were explored in this article.Three kinds of media were screened by the flask experiments,and a semi-synthetic medium which has the potential of high-density fermentation as the pilot scale medium was selected in the fermentor.The culturation temperature,dissolved oxygen,pH,rotational velocity,and feeding methods etc.were optimized,the E.coli density of fermentation is up to OD600 22.92 with the semi-synthetic medium,and the humid weight of engineering bacteria is 27.173 g/L.The outcome of plasmid is 1.44 mg/g.The stability of the plasmid in the engineering bacteria was also verification.

关 键 词：牛分支杆菌 三价DNA疫苗 半合成培养基 发酵工艺 

分 类 号：S858.23[农业科学—临床兽医学] S852.618[农业科学—兽医学]