miR-221异常表达对胃癌细胞增殖和凋亡的影响  

Effects of abnormal expression of miR-221 on proliferation and apoptosis of gastric cancer cell lines

作　　者：陶春华[1] 苏婧玲[1] 黄志刚[1] 刘占举[1] 

机构地区：[1]同济大学附属第十人民医院胃肠内科,上海200072

出　　处：《同济大学学报（医学版）》2010年第2期44-46,54,共4页Journal of Tongji University(Medical Science)

摘　　要：目的探讨miR-221反义寡核苷酸(miR-221 ASO)对胃癌细胞增殖的调控作用。方法转染miR-221ASO,MTT实验观察miR-221 ASO对胃癌细胞调控产生的系列效应,采用茎环RT-PCR检测胃癌细胞株SGC-7901和MKN-45中miR-221的表达变化。结果 miR-221 ASO转染可显著抑制胃癌细胞SGC-7901和MKN-45表达(P=0.027、0.013),miR-221 ASO对胃癌细胞的生长抑制率显著减少。结论 miR-221 ASO对胃癌细胞miR-221水平降低具有靶向特异性,转染miR-221 ASO可有效抑制miR-221的促癌效应,利用反义核酸技术的高度特异性开展针对促癌miRNA分子的靶向治疗将可能为胃癌的基因治疗开辟新的途径。Objective To explore the role played by microRNA-221 on the regulation, of gastric cancer cell proliferation by using antisense MiR-221 ASO. Methods MiR-221 ASO was transfected into gastric cancer cells via liposome,the blocking effect was verified by the down-regulation of MMP7 expression examined by RT-PCR. The effect of miR-221 ASO on the growth of cancer cell lines in vitro was analyzed by MTT assay. Results The expression of miR-221 was down-regulated significantly in gastric cancer cells transfected with miR-221 ASO. Furthermore,the growth of gastric cancer cell lines was inhibited through down-regulating miR-221 ASO. Conclusion The promoting activities of miR-221 on gastric cancer cells can be antagonized effectively by miR-221 ASO. The use of antisense nucleic acid technology for gene therapy of gastric cancer may hold great promise.

关 键 词：微小RNA 胃肿瘤 靶向治疗 

分 类 号：R735.2[医药卫生—肿瘤]