肝细胞肝癌和肝硬化组织中丙型肝炎病毒各区段抗原的检测  

Detection of hepatitis C virus-C, E, NS3 and NS4 regions' antigens in hepatocellular carcinoma and liver cirrhosis

作　　者：王理富[1,2,3] 李德富[1,2,3] 郎淑慧[1,2,3] 尹红章 冯建平[1,2,3] 李冠群 姜卫国[1,2,3] 赖文敏 

机构地区：[1]中国药品生物制品检定所卫生部生物技术产品检定及标准化重点实验室 [2]中国医科大学附二院病理科 [3]北京医院病理科

出　　处：《中华实验和临床病毒学杂志》1998年第4期326-329,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家"九五"科技攻关课题基金

摘　　要：目的研究丙型肝炎病毒（ＨＣＶ）在肝细胞肝癌（ＨＣＣ）和肝硬化（ＬＣ）中所起的作用，结合乙型肝炎病毒（ＨＢＶ）进行分析，并初步探讨ＨＣＶ与ＨＢＶ感染是否有相互促进作用。方法采用ＨＣＶ－Ｃ、Ｅ、ＮＳ３、ＮＳ４区单克隆抗体、ＨＢｓＡｇ多克隆抗体用免疫组化方法检测了５９例ＨＣＣ及３５例ＬＣ组织标本。结果ＨＣＶ阳性反应主要分布在肝细胞及癌细胞的胞浆内，呈细颗粒状。ＨＣＣ中，ＨＣＶ感染率：北京（２９例）为１７２％、沈阳（３０例）为２６７％；沈阳３５例ＬＣ肝组织病人中ＨＣＶ感染率１４３％。各区段单抗单独检测以Ｃ区单抗检测阳性率最高。乙肝表面抗原的检测阳性率：北京ＨＣＣ（２９例）为６３０％，沈阳ＨＣＣ（３０例）为７３３％，沈阳ＬＣ（３５例）为５４３％，均明显高于各自的ＨＣＶ抗原检测阳性率。结论ＨＣＶ在ＨＣＣ、ＬＣ中起一定作用，且Ｃ区抗原可能在ＨＣＣ中表达率较高；ＨＢＶ、ＨＣＶ感染在ＨＣＣ、ＬＣ中无明显相互促进作用。Using 4 McAbs to HCV-C, E, NS3 and NS4 regions' antigens and PcAb to HBsAg, 59 cases of hepatocellular carcinoma(HCC) and 35 cases of liver cirrhosis(LC) were tested by immunohistochemistry technique. Positive reactions for hepatitis C virus were mainly present in the cytoplasm of hepatocytes and tumor cells with fine granules. The positive rates of HCV were 17 2% in HCC(29 cases) of Beijing, 26 7% in HCC(30 cases) of Shenyang and 14 3% in LC(35cases) of Shenyang. C region's McAb had the highest positive rate of detection, which suggested that C region's protein had a high level expression. The positive rates of hepatitis B virus surface antigen were 63 0% in HCC(29 cases) of Beijing, 73 3% in HCC(30 cases) of Shenyang and 54 3% in LC(35 cases) of Shenyang, all of which were higher than their positive rate for HCV detected. In HCC and LC, HBV and HCV inclined to suppress the opposite side.

关 键 词：肝细胞肝癌 肝硬化 免疫组化 单克隆抗体 HBSAG 

分 类 号：R735.7[医药卫生—肿瘤]