产酯化脂肪酶微生物的筛选研究  

Screening of Lipase-producing Microorganisms

作　　者：梁晓夏[1] 黄佳箭[1] 戚启琼[1] 李日伟[1] 梁静娟[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005

出　　处：《现代食品科技》2010年第5期509-511,462,共4页Modern Food Science and Technology

摘　　要：用罗丹明B透明圈法从广东、广西和海南采集的土样中筛选到658株透明圈比较明显的菌株,经发酵测定脂肪酶酶活,获得15株脂肪酶活力超过7U/mL的菌株,采用固定化脂肪酶粗酶制剂的方法进行酯化能力的测定,得到产生酯化脂肪酶能力较强的3个菌株GX-20、GX-35和GX-53,它们的酯化率分别为18.12%、20.65%和19.72%。其中的GX-35菌株经16SrDNA基因序列分析鉴定为Burkholderia cepacia。Six hundred and fifty eight stlains of microorganisms with lipase-producing capability were obtained using Rhodanmine B plate. The lipase activities of 15 strains were more than 7 U/mL. The capacity of the 15 strains in catalyzing transesterification was determined by alkali titration assay and the methods of immobilized crude enzyme. Three strains GX-20, GX-35 and GX-53 showed higher transesterification activity than the others. The transesterification rates by the three strains were 18.12%, 20.65% and 19.72%, respectively. GX-35 was identified as Burkholderia cepacia according to 16S rDNA gene sequence analysis.

关 键 词：酯化脂肪酶 罗丹明B透明圈法 16S RRNA序列 

分 类 号：TQ925[轻工技术与工程—发酵工程]