PRK与LASIK术后角膜组织神经肽P物质表达的研究  

Expression of neuropeptide substance P in corneal tissues after PRK and LASIK

作　　者：郑磊[1] 雷蕾[1] 张建华[1] 高鹏[1] 王倩[1] 樊琪[1] 冯华章[1] 王红英[1] 

机构地区：[1]第二军医大学附俑长海医院眼科,上海市200433

出　　处：《眼科新进展》2010年第5期427-429,共3页Recent Advances in Ophthalmology

摘　　要：目的定量检测神经肽P物质(substance P,SP)在准分子激光角膜切削术(photorefractive keratectomy,PRK)及准分子激光原位角膜磨镶术(laser in-situ keratoleusis,LASIK)术后角膜中的表达水平,探讨SP在PRK术后组织愈合中的作用。方法12只新西兰白兔,左眼行PRK,右眼行LASIK,手术均按近视-4.00D施行,切削光学消融直径6mm,切削深度80μm,于术后1个月取角膜组织,提取总RNA,逆转录cDNA,采用荧光定量RT-PCR方法,分别检测角膜组织SP的表达水平。结果PRK与LASIK术后角膜组织SP表达水平不同,PRK术后SP mRNA平均相对含量为0.024120±0.002681(SP/β-actin),LASIK术后平均相对含量为0.002500±0.000353(SP/β-actin),PRK术后明显高于LASIK术后,二者比较差异有显著统计学意义(P=0.000)。结论PRK和LASIK术后角膜组织中SP表达水平不同,SP在PRK术后角膜组织中的表达水平增高,可能参与调控PRK术后组织的愈合过程。Objective To quantitatively detect the expression of neuropeptide substance P（SP）in cornea after photorefractive keratectomy（PRK）and laser in-situ keratomileusis（LASIK）,and to discuss the effect of SP during corneal healing after PRK.Methods LASIK was performed on the right eyes and PRK on the left eyes in twelve rabbits,all surgery were carried with-4.00 D myopia,the lighting diameter of artherectomy was 6 mm,and the depth of artherectomy was 80 μm.The corneal tissues were taken at 1 month after ablation,the total RNA was made,and cDNA was reverse transcribed,the expressive level of SP was detected by quantitative RT-PCR.Results The expressive levels of SP in cornea varied after PRK and LASIK,the mean relative content of SP mRNA after PRK was 0.024 120±0.002 681（SP/β-actin）,which after LASIK was 0.002 500±0.000 353（SP/β-actin）,the former was higher than the latter,there was significant difference（P=0.000）.Conclusion The expressive level of SP in cornea varies after PRK and LASIK,the SP expression increases in the corneal tissue after PRK,which indicates that SP may involved in regulating the process of corneal healing after PRK.

关 键 词：准分子激光角膜切削术 准分子激光原位角膜磨镶术 角膜 P物质 

分 类 号：R779.63[医药卫生—眼科]