神经生长因子诱导基因B-β在大鼠视网膜无长突细胞发育过程中的表达  

Expression of nerve growth factor-induced gene B-β in development of rat retinal amacrine cells

作　　者：李英敏[1] 马春玲[1] 左敏[1] 韩业兴[1] 马如飞[1] 毕海涛[1] 杨莹[1] 丛斌[1] 

机构地区：[1]河北医科大学基础医学院,河北省石家庄市050017

出　　处：《眼科新进展》2010年第5期434-437,共4页Recent Advances in Ophthalmology

基　　金：人事部回国留学人员择优项目基金资助(编号:2009010)~~

摘　　要：观察孤儿核受体神经生长因子诱导基因B-β（nerve growth factor-induced gene B-β,NGFIB-β）在大鼠视网膜发育过程中蛋白表达的动态变化及与无长突细胞的相关关系。方法采用孕14d、16d、18d妊娠大鼠（各5只）、生后0d、3d、5d、6d、7d、9d、13d、15d（各5只）的幼鼠及成熟大鼠（5只）,全部处死后立刻取出眼球组织于福尔马林中固定,石蜡包埋、组织切片,免疫组织化学及免疫组织化学双重染色后显微镜下观察。结果NGFIB-β在视网膜发育过程中的表达出现了显著的动态变化。NGFIB-β首先在孕18d胎鼠视网膜组织出现少量表达,阳性表达主要在内核层内缘,随着发育的进行阳性细胞数目明显增多,生后3-7d达到高峰,之后随着细胞的成熟阳性表达又逐渐减少,成熟的视网膜组织内仅见少量阳性细胞,通过NGFIB-β和视网膜无长突细胞特异性标记物突出融合蛋白-1的免疫组织化学双重染色显示NGFIB-β阳性细胞为无长突细胞,两种蛋白共同表达在一个细胞内。结论NGFIB-β在大鼠视网膜无长突神经细胞的分化成熟过程中起重要作用。To observe the dynamic change of the protein expression of orphan nuclear receptor nerve growth factor-induced gene B-β（NGFIB-β）in the development of rat retina,and its relationship with the amacrine cells.Methods The embryonate 14 days（E14）,16 days（E16）,18 days（E18）rats（each 5 cases）and postnatal 0 day（P0）,3 days（P3）,5 days（P5）,6 days（P6）,7 days（P7）,9 days（P9）,13 days（P13）,15 days（P15）rats（each 5 cases）were chosen and sacrificed,the eyeballs were enucleated and fixed in formalin,then paraffin-embedded tissue sections were made,the immunohistochemical staining and immunohistochemical double staining were adopted,the results were observed under microscope.Results The expression of NGFIB-β in the development of retina had the obvious dynamic changes.NGFIB-β was firstly expressed in small quantities in the E18 rat retinal tissues,positive expressions were mainly at the inner margin of inner nuclear layer,the positive cells increased with the development of retina and peaked from P3 to P7,then gradually decreased with the maturation of cells,only few were observed in the matured retinal tissues.The immunohistochemical double staining with NGFIB-β and Syntaxin-1（HPC-1,a specific amacrine cell marker of retina）showed that the NGFIB-β positive cells were amacrine cells,the two proteins coexpressed in the same cell.Conclusion NGFIB-β may play an essential role during the differentiation and maturation of the amacrine cells of rat retina.

关 键 词：神经生长因子诱导基因B-β 孤儿核受体 无长突细胞 分化 

分 类 号：R744.1[医药卫生—神经病学与精神病学]