超高效液相色谱法对塑料玩具中16种环境雌激素的同时测定  

Simultaneous determination of sixteen kinds of EEs in plastic toys by UPLC

作　　者：徐靖[1] 陈兴宇[1] 朱静波[1] 汪欢晃 

机构地区：[1]国家日用小商品质量监督检验中心,浙江义乌322000

出　　处：《生态环境学报》2010年第4期826-829,共4页Ecology and Environmental Sciences

基　　金：浙江省分析测试科技计划项目(2009F70051);浙江省质监系统定向委托科研项目(20080503)

摘　　要：含有环境雌激素的塑料玩具及儿童用品有可能被小孩放进口中,如果放置的时间足够长,就会导致雌激素的溶出量超过安全水平,危害儿童的肝脏和肾脏,也可引起儿童性早熟。采用超高效液相色谱法,对塑料玩具中16种环境雌激素类化合物进行检测,建立了超高效液相色谱法同时测定塑料玩具中16种环境雌激素的方法。采用超生萃取法进行提取,使用乙醇作为提取溶剂,在前处理过程中对样品进行无水硫酸钠脱水处理,使杂质显著降低;以乙腈-水为流动相,考察了5种具有不同选择性的UPLC色谱柱对16种环境雌激素的分离效果,结果表明,16种环境雌激素在ACQUITY UPLC BEH C18色谱柱上获得了最为理想的分离效果。该方法的回收率为93.6%～98.8%,RSD为0.5%～2.3%,检出限达0.11～0.76 ng。采用本方法对市售的28种儿童塑料玩具进行了检测,检出的环境雌激素有DEHP、DCHP、DMP、DNOP,其它未检出,其中DEHP、DCHP和DMP检出率相对较高,分别占32.8%、24.5%、19.5%,DNOP则最小,检出率为10.4%。此方法简便、易行、分离度较好,可作为测定塑料玩具中增塑剂环境雌激素类化合物的方法。Children possibly put plastic toys and children products containing environmental estrogen hormone in mouth,if this peirod is long enough,it will lead the release of environmental estrogen hormone exceeds to the safety level and harm to the children’s liver and kidney.Finally it will lead sexual precocity.This paper uses high performance liquid chromatography to make in-spection for 16 kinds of environmental estrogen hormone compounds in plastic toys and sets the method of using high performance liquid chromatography to make inspection simultaneous for 16 different kinds of environmental estrogen hormone.We use ultrasonic wave extracted method to extract and use ethanol as the solvent.In pretreatment progress,we make sodium sulphate anhydrous dehydration for samples to purify,and use acetonitrile-water as mobile phase to research separation effect of 16 kinds of environmental estrogen hormone on five selective UPLC column,finally we find the ideal separate effect of 16 kinds of environmental estrogen hormone appearing in ACQUITY UPLC BEH C18 column.The recovery varied from 93.6% to 98.8%,the relative standard derivation ranged from 0.5% to 2.3%.The limit of detction ranged from0.11 to 0.76ng.We make inspection for 28 kinds of plastic toys in the sale and detect environmental estrogen hormone conclding DEHP,DCHP,DMP,DNOP.And the detect rate of DEHP,DCHP and DMP are relatively higher,respectively accounting for 32.8%,24.5%,19.5%.The detect rate of DNOP is minimum,is 10.4%.this method is convenient,simple and easy to use,and have good separate effect,so it is a good choice to determine the environmental estrogen hormone compounds in plastic toys’ plasticizer.

关 键 词：超高效液相色谱 塑料玩具 环境雌激素 

分 类 号：X132[环境科学与工程—环境科学]