E-钙黏蛋白及黏着斑激酶在直肠癌组织中的表达及意义  

Significance and expression of E-cadherin and focal adhesion kinase in rectal cancer

作　　者：张险峰[1] 江应安[1] 于皆平[1] 于红刚[1] 

机构地区：[1]武汉大学人民医院消化内科,430060

出　　处：《中华消化外科杂志》2010年第2期143-146,共4页Chinese Journal of Digestive Surgery

基　　金：国家自然科学基金（30300154）

摘　　要：目的探讨E-钙黏蛋白（E—CD）及黏着斑激酶（FAK）和磷酸化黏着斑激酶（FAKpy397）在直肠癌组织中的表达水平及其临床意义。方法收集2001年至2002年武汉大学人民医院手术切除的直肠癌及对应癌旁组织石蜡标本30例和2006年至2007年手术切除的直肠癌新鲜标本及对应癌旁组织35例，应用免疫组织化学法、Western blot检测E—CD、FAK和FAKpy397的表达，采用X^2检验分析相关数据。结果正常直肠黏膜中E—CD呈细胞膜阳性表达，直肠癌组织中E—CD细胞膜表达缺失，直肠癌组织中E—CD、FAK及FAKpy397的表达与肿瘤分化程度、浸润深度和淋巴结转移有关（X^2=7．099、18．358、25．612，12．316、28．823、23．168，8．927、18．122、22．620，P〈0．05）；与年龄、性别无关（X^2=0．439、1．899、3．676，0．541、4．051、1．135，P〉0．05）；癌组织和癌旁组织中FAK及FAKpy397表达率分别为83％（54／65）、68％（44／65）和31％（20／65）、26％（17／65），两者比较差异有统计学意义（X^2=33．707，34．163，20．897，P〈0．05）。结论直肠癌组织中E—CD细胞膜表达缺失、FAK和FAKpy397的表达水平明显升高，与肿瘤分化程度、浸润深度和淋巴结转移有关，可预测肿瘤的生物学行为。Objective To investigate the significance and expression of E-cadherin （E-CD）, focal adhesion kinase （FAK） and FAK py397 in rectal cancer. Methods Thirty paraffin specimens of rectal cancer and adjacent tissues collected from 2001 to 2002 and 35 fresh specimens of rectal cancer and adjacent tissues resected from 2006 to 2007 at People＇s Hospital of Wuhan University were adopted in this study. The expression of E-CD, FAK and FAK py397 was detected by immunohistochemistry and Western blot. All data were analyzed using chi-square test. Results E-CD was positively expressed in the membranes of epithelial cells in normal rectal mucosa, while no E-CD expression was found in the membranes of epithelial ceils in rectal cancer tissues. There was a significant correlation between the expression of E-CD, FAK, FAK py397 and tumor differentiation, depth of invasion and lymph node metastasis （X^2 = 7. 099, 18. 358, 25. 612; 12. 316, 28. 823, 23. 168; 8. 927, 18. 122, 22. 620, P 〈 0.05） , while no relationship between the expression of E-CD, FAK, FAK py397 and age, sex was detected （X^2 =0.439, 1.899, 3. 676; 0.541, 4. 051, 1.135, P〉0.05）. The expression levels of FAK and FAK py397 were 83% （54/65） and 68% （44/65） in rectal cancer tissues, and were 31% （20/65） and 26% （17/65） in adjacent tissues, with significant difference （X^2 = 33. 707, 34. 163, 20. 897, P 〈 0. 05 ）. Conclusion Lost expression of E-CD in the membrane of rectal cancer tissue and elevated expression of FAK and FAK py397 in the rectal cancer tissue might be related to the invasion and metastasis of rectal cancer, which may predict the biological behavior of rectal cancer.

关 键 词：直肠肿瘤 E-钙黏蛋白 黏着斑激酶 

分 类 号：R735.37[医药卫生—肿瘤]