CPC/氨磷汀复合体理化性能和体外细胞学实验研究  

In Vitro Study on Physical and Chemical Properities of Calcium Phosphate Cement/amifostine Complex and on Vitality of Cultured Cells

作　　者：刘彦宁[1] 刘淼[1] 刘娟[1] 任鹏宇[2] 

机构地区：[1]西安交通大学第一附属医院骨科,西安710061 [2]陕西省人民医院骨科,西安710061

出　　处：《生物医学工程学杂志》2010年第2期337-341,共5页Journal of Biomedical Engineering

基　　金：陕西省科学技术研究发展计划项目资助(2007K09-09)

摘　　要：对复合有氨磷汀的磷酸钙骨水泥用作骨缺损填充材料进行基础性研究。本研究采用混合-模压法将不同质量比的氨磷汀载入磷酸钙骨水泥制备磷酸钙骨水泥/氨磷汀复合体,分别对其固化时间、力学强度、孔隙率、电镜扫描、对骨肉瘤细胞、血管内皮细胞是否具有保护作用进行观察。研究表明磷酸钙骨水泥中载入0.1%和0.5%氨磷汀不影响磷酸钙骨水泥的固化时间、强度、孔径、孔隙率,对骨肉瘤细胞不具有保护作用,不影响血管内皮细胞的增殖分化。因此在磷酸钙骨水泥中载入0.1%和0.5%氨磷汀在肿瘤性骨缺损的化学治疗中可能是有意义的。This study was designed to assess the feasibility of calcium phosphate cement/amifostine complex as a new material for filling the bone defect caused by tumor resection.Mixed-molding method was used,the mass ratios of 0%,0.1%,0.5%,1%,2% of amifostine/calcium phosphate cement complex being adopted.The curing time,mechanical strength,porosity,scanning electron micrograph,osteosarcoma cells＇ vitality and vascular endothelial cells＇ vitality relevant to the complex in vitro were observed.Calcium phosphate cement being loaded with 0.1% and 0.5% amifostine did not affect the curing time,strength,pore size and porosity of calcium phosphate bone cement.In addition proliferation and differentiation of osteosarcoma cells and vascular endothelial cells were not affected.These data suggest that phosphate cement containing 0.1% and 0.5% amifostine be of significance in the treatment regimen as bone defect filling materials..

关 键 词：磷酸钙骨水泥 氨磷汀 骨肉瘤细胞 血管内皮细胞 

分 类 号：R738.1[医药卫生—肿瘤]