大鼠慢性高眼压视网膜Nogo-A的表达(英文)  

Expression of Nogo-A on the retina in rat model with chronic ocular hypertension

作　　者：聂庆珠[1] 刘致力[1] 沙倩 高殿文[1] 

机构地区：[1]中国医科大学附属盛京医院眼科,中国辽宁省沈阳市110004 [2]黑龙江省眼病医院,中国黑龙江省哈尔滨市150001

出　　处：《国际眼科杂志》2010年第5期825-826,共2页International Eye Science

基　　金：Scientific and Technological Research Funded Projects in Liaoning Province,China(No.2009225021)~~

摘　　要：目的:研究慢性高眼压大鼠视网膜髓鞘相关抑制蛋白No-go-A表达的变化。方法:成年雄性Wistar大鼠36只随机分为正常对照组6只和慢性高眼压组30只,应用免疫组织化学方法观察慢性高眼压大鼠视网膜不同时点Nogo-A表达的变化。结果:与正常对照组相比,慢性高眼压21d大鼠视网膜变薄,节细胞数量减少(P<0.05);Nogo-A表达增多,与形态学变化相一致(P<0.05)。结论:髓鞘相关抑制蛋白Nogo-A在慢性高眼压大鼠视网膜损伤过程中发挥了重要作用。AIM： To study the expressive variation of Nogo-A on rat retina in the process of chronic ocular hypertension. METHODS： Thirty-six healthy adult male Wistars were randomly divided into control group （6 rats） and chronic hypertension group （30 rats）. Chronic hypertension was created by cauterizing the superficial scleral veins. Immunohistochemistry technique was used to evaluate the expressive varieties of Nogo-A at different time points during the course of chronic ocular hypertension. RESULTS： The success of the model was indicated by over 40% of increase in the IOP as compared with normal rats. Compared with control group, as time passed chronic hypertension group gradually had detectable morphology changes in the retina. At the 21st day of chronic ocular hypertension, retinas became thinner and the quantity of retinal ganglion cell （RGC） decreased （P〈0.05）. Assoicated with the morphological changes, the expression of Nogo-A was strongly increased （P〈0.05）. CONCLUSION： Myelin associated protein Nogo-A plays a part in the process of chronic ocular hypertension.

关 键 词：视网膜 慢性高眼压 NOGO-A 视网膜神经节细胞 

分 类 号：R774.1[医药卫生—眼科]