成人结膜干细胞定位特征的免疫组织化学研究  

Immunohistochemical study on localization characteristics of conjunctival epithelial stem cells in adult

作　　者：裴涌[1] 洪晶[2] 张昊[1] 郭嘉术[1] 

机构地区：[1]中国辽宁省沈阳市第四人民医院眼科,110031 [2]北京大学附属第三医院眼科,中国北京市100083

出　　处：《国际眼科杂志》2010年第5期853-855,共3页International Eye Science

摘　　要：目的:研究成人结膜上皮细胞中,粘蛋白5AC(MUC5AC),角蛋白7,p63和增殖细胞核抗原(PCNA)的表达及分布情况,探讨成人结膜上皮干细胞的定位特征。方法:获取角膜移植供者的睑球结膜,采用HE免疫组化染色法,观察MUC5AC,角蛋白7,p63和PCNA的表达及分布情况。结果:PCNA及p63表达情况一致,主要表达在睑缘皮肤黏膜交界处及近角膜缘结膜上皮深层细胞。角蛋白7在穹窿部球结膜的固有层中的腺体细胞有阳性表达,MUC5AC在各部位结膜上皮的杯状细胞的胞质阳性表达。结论:MUC5AC细胞角蛋白7和p63及PCNA在成人结膜上皮细胞的特征性表达情况可以推测具有分化潜力的结膜干细胞存在于睑缘皮肤黏膜交界处及近角膜缘球结膜上皮深层细胞。AIM：To study the expression and distribution of MUC5AC, keratin 7, p63 and proliferating cell nuclear antigen（PCNA） of adult conjunctival epithelial cells to investigate the localization characteristics of adult conjunctival epithelial stem cells. METHODS： Conjunctiva was collected from donor of corneal transplantation. HE immunohistochemical staining were performed to observe the expression and distribution of MUC5AC,keratin 7, p63 and PCNA. RESULTS： PCNA and p63 staining showed similar expression pattern,mainly in the deep layer nucleus of palpebral mucocutaneous junction and limbal region. Keratin 7 was positive expressed in the gland cells of lamina propria in the fornix region. MUC5AC was positive expressed in the cytoplasm of goblet cells in various parts of conjunctival epithelium. CONCLUSION：Conjunctival stem cells with high capability of proliferation are mainly located in mucocutaneous junction area and limbus in adult.

关 键 词：结膜上皮干细胞 定位 免疫组织化学 

分 类 号：R770.2[医药卫生—眼科]