大鼠皮肤肌肉切口牵拉术致持续性疼痛的动物模型  

A rat model of persistent postoperative pain evoked by skin/muscle incision and retraction

作　　者：范素贞[1] 胡兴国[2] 曾因明[1] 

机构地区：[1]江苏省麻醉学重点实验室,徐州221002 [2]湖南省桃源县人民医院麻醉科

出　　处：《国际麻醉学与复苏杂志》2010年第2期119-122,共4页International Journal of Anesthesiology and Resuscitation

基　　金：湖南省科技计划一般项目资助课题（2009SK3018）

摘　　要：目的建立大鼠皮肤肌肉切口牵拉（skin／muscle incision and retraction，SMIR）术后持续性疼痛模型，研究SMIR术对术后疼痛的影响。方法雄性SD大鼠36只，采用随机数字表法将大鼠随机分为空白对照组、假手术组和模型组，每组12只。按Flatters法制作SMIR持续性疼痛模型，应用von Frey纤维细丝法和Hargreaves法测定术前及术后第1、3、7、12、22、32天的机械缩足反射阈值（mechanical withdrawal threshold，MWT）和热缩足潜伏期（thermal withdrawal latency，TWL）；并且每组采用随机数字表法随机取4只于术后12d时电镜下观察手术侧隐神经损伤情况。结果与空白对照组，模型组大鼠MWT在术后第1天即出现降低（P=0．015〈0．05）；12d时下降最为显著（P=0．001〈0．01），32d时差异无统计学意义（P=0．772〉0．05）；而假手术组与空白对照组比较在各时间点MWT差异无统计学意义；整个观察时间过程中模型组大鼠TWL均无明显改变；SMIR术及假手术后隐神经均未出现髓鞘肿胀或脱髓鞘等神经损伤现象。结论进一步证实了SMIR模型的稳定性和可重复性，将为研究术后持续性疼痛的机制及治疗措施提供一个新的平台。Objective To establish a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction （SMIR） and to test its efficacy. Methods Thirty-six male SD rats were randomly divided into three groups （n=12）： control group, sham group and model group. SMIR was produced as Flatters described and the threshold with von Frey filament and radiant heat stimulation were measured before operation （baseline） and at 1st, 3rd, 7th, 12th, 22nd and 32rid postoperative day. The structural damages to myelin sheath of saphenous nerve in 4 rats of each group randomly were observed with electron microscope at 12th postoperative day. Results SMIR surgery induced signi？cantly persistent mechanical hypersensitivity to yon Frey stimulation of the plantar ipsilateral hindpaw. Compared to the responses of control group rats, the threshold to mechanical stimuli started to decrease at 1 d （P=-0.015〈0.05） and peaked at 12 d （P=0.001〈0.01） after operation. There was no significant difference between the SMIR group and control group at 32 d after surgery （P=0.772〉0.05）. Mechanical sensitivity of control group and sham group did not significantly change. SMIR did not induce significant heat hyperalgesia. Electron microscopy of saphenous nerve sections did not show demyelination or oedema in the saphenous nerve of the model group or sham group. Conclusion The results of the present study suggest that the model of persistent postoperative pain evoked by SMIR is stable and repeatable, which may provide a new stage for the study on the mechanisms and treatment of postoperative pain.

关 键 词：术后持续性疼痛 动物模型 疼痛行为学 

分 类 号：R-332[医药卫生] R619