日本圆叶海棠组培苗的快速繁殖  

Rapid Propagation of Tissue Culture Seedling of the Japanese Malus prunifolia var. ringo

作　　者：陈宗礼[1] 高彦[2] 刘娜 齐向英[1] 李霞[1] 张向前[1] 李宁[1] 

机构地区：[1]延安大学生命科学学院陕西省区域生物资源保育与利用工程技术研究中心,陕西延安716000 [2]陕西省果树良种苗木繁育中心,陕西铜川727031 [3]陕西省延安中学,陕西延安716000

出　　处：《西北农业学报》2010年第2期183-188,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省教育厅重点实验室项目资助

摘　　要：以日本圆叶海棠初代培养获得的茎段为外植体,采用正交和随机区组设计培养基进行继代和生根培养。建立了日本圆叶海棠试管苗快繁体系,并优化获得其适宜继代的培养基MS+IBA 0.2 mg/L+6-BA0.3 mg/L+蔗糖3%+琼脂0.55%,适宜的生根培养基为1/2MS+IAA 0.5 mg/L+蔗糖2%+琼脂0.55%。研究表明,MS基本培养基附加低浓度水平的6-BA与IBA的激素组合,有利于丛生芽的诱导,1/2MS基本培养基附加低浓度的IAA,有利于根的诱导,NAA与IBA不适用于生根。生根苗的移栽可采用温室自然光过渡法炼苗后,以蛭石为基质的营养钵移栽法。Taken the original cultured stem sections of Japanese Malus prunifolia var.ringo as explants,the mediums for subculture and rooting culture were designed by using the orthogonal and the randomized block experiments.Then,the rapid propagation system of tube seedling of Japanese Malus prunifoliavar.ringo was well established.The results showed that the suitable medium for subculture was composed of MS ＋ IBA 0.2 mg/L ＋ 6-BA 0.3 mg/L ＋ sucrose 3% ＋ agar 0.55%,and medium for rooting culture was composed of 1/2MS ＋ IAA 0.5 mg/L ＋ sucrose 2% ＋ agar 0.55%.It indicated that the addition of the low concentration of 6-BA and IBA to MS basic medium is advantageous to the induction of axillary bud.Moreover,1/2MS basic medium combined with the low concentration of IAA was useful to the rooting induction.However,NAA and IAA were not suitable for the rooting culture.Transplanting of rooting seedlings may use the method of greenhouse natural light transition to build up the seedling after,use the nutrition earthen bowl transplants in greenhouse take the vermiculite as the matrix.

关 键 词：日本圆叶海棠(Malus prunifolia VAR. ringo) 茎段快繁 继代与生根培养 生根苗移栽 

分 类 号：S685.99[农业科学—观赏园艺]