肌酸六磷酸对裸鼠皮下移植HT-29细胞瘤的生长抑制作用研究  

STUDY ON THE INHIBITORY EFFECT OF INOSITOL HEXAPHOSPHATE ON THE GROWTH OF SUBCUTANEOUS TRANSPLANTED TUMOR OF HT-29 CELL IN NUDE MICE

作　　者：李敏[1] 宋扬[1] 

机构地区：[1]青岛大学医学院医学营养研究所,青岛266021

出　　处：《营养学报》2010年第2期121-125,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30671767);山东自然科学基金(No.2007BS03042)

摘　　要：目的研究肌醇六磷酸(IP6)对HT-29细胞裸鼠皮下移植瘤生长的抑制作用。方法建立HT-29细胞裸鼠移植瘤模型,随机分为4组,对照组,5-氟尿嘧啶组(5-FU)组,IP6低剂量组,IP6高剂量组。每组8只。每组每日腹腔注射1次0.5ml;分别含生理盐水,5-FU20mg/kgbw,IP640mg/kgbw,100mg/kgbw。用药20d后经颈椎脱位处死。比较各组抑瘤作用和裸鼠一般状态的变化,对标本分别行光镜和电镜观察、Hoechst染色、原位末端标记(TUNEL)和免疫组化检测。结果生理盐水,低、高IP6及5-FU组瘤重分别为0.68±0.31、0.57±0.28、0.58±0.18、0.24±0.23g;瘤体积分别为0.71±0.35、0.58±0.21、0.59±0.20和0.34±0.27cm3;与对照组比,5-FU组瘤量、瘤体积显著降低(P<0.05),IP6组瘤重和体积呈一定降低趋势,但无显著差异(P>0.05)。免疫组化检测显示,与对照组比,低、高IP6组P53蛋白表达显著降低(P<0.05),PCNA表达无显著差异(P>0.05)。TUNEL检测结果,与对照组比,低、高IP6组细胞凋亡率显著升高(P<0.05)。结论IP6可能通过抑制P53表达、诱导肿瘤细胞凋亡,而起到抑制和延缓肿瘤生长的作用。Objective To investigate the inhibitory effect of inositol hexaphosphate on the growth of subcutaneous transplanted tumor of HT-29 cell in nude mice. Method Established models of subcutaneous transplantable HT-29 cell tumor in nude mice and the mice were divided randomly into four groups：control group,5-fluorouiaical（5-FU） group,low-dose IP6 group,high dose IP6 group,eight animals per each group,0.5 ml containing normal saline,5-FU 20 mg/kg bw,IP6 40 mg/kg bw and IP6 100mg/kg bw was administered i.p. respectively to above groups once daily. After continuous administration for 20d,the mice were killed. The anti-tumor effect in each group was observed and Specimens were detected by light microscopy and electron microscopy,Hoechst staining,TUNEL and immunohistochemical method. Results The tumor weights and volumes in control group,low-dose IP6 group,high-dose IP6 group and 5-FU group were 0.68±0.31,0.57±0.28,0.58±0.18,0.24±0.23g. and 0.71±0.35,0.58±0.21,0.59±0.20,0.34±0.27 cm^3 respectively. Compared with the control group,the weight and volume of tumors in 5-FU group were decreased significantly （P〈0.05）. The weight and volume of tumors in IP6 groups showed a reduction trend,but not significant statistically. Compared with the control group,P53 protein expression in low-dose and high-dose IP6 groups was significantly reduced （P〈0.05）,but PCNA protein expression was not different significantly. TNUEL detection showed that apoptosis rate in IP6 groups were significantly higher than control group （P〈0.05）. Conclusion IP6 could inhibit the growth of HT-29 cell implanted tumor mildly through inducing cell apoptosis and reducing expression of P53.

关 键 词：肌醇六磷酸 结肠癌 HT-29细胞系 裸鼠 移植瘤 

分 类 号：R73-3[医药卫生—肿瘤]