雌马酚对人卵巢癌SKOV-3细胞增殖的抑制作用及其机制研究  

THE INHIBITORY EFFECT OF EQUOL ON HUMAN OVARIAN CARCINOMA CELL SKOV-3 PROLIFERATION AND ITS MECHANISM

作　　者：任国峰[1] 杨爱青[1] 杨丽娜[1] 朱明元[1] 黄忆明[1] 

机构地区：[1]中南大学公共卫生学院,长沙410078

出　　处：《营养学报》2010年第2期174-177,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30500407)

摘　　要：目的观察雌马酚(equol)对人卵巢癌SKOV-3细胞增殖的抑制作用,并探讨其作用的分子机制。方法用不同浓度(10-8、10-7、10-6、10-5、5×10-5、10-4mol/L)雌马酚处理SKOV-3细胞24、48、72h后,及同时用10μmol/LERK通路抑制剂U0126或雌激素受体抑制剂ICI182,780处理1h后再用50μmol/L雌马酚处理2h,测定各组SKOV-3细胞存活率,用Westernblotting检测总ERK和磷酸化ERK1/2(p-ERK)的蛋白表达水平。结果各实验组SKOV-3细胞存活率随着雌马酚作用浓度和时间的增加而降低,总ERK蛋白表达量不变,p-ERK的表达量随雌马酚处理浓度的增加而逐渐降低。U0126、ICI182,780单独使用或与雌马酚联合使用均能够降低p-ERK的蛋白表达。结论雌马酚显著抑制人卵巢癌SKOV-3细胞的增殖,其机制是通过下调磷酸化ERK蛋白的表达发挥作用。Objective To investigate the effect of equol on ERK mediated signal transduction pathway and to clarify its mechanism of proliferation inhibition on human ovarian carcinoma cell line SKOV-3. Method SKOV-3 cells were treated with 10^-8,10^-7,10^-6,10^-5,5×10^-5,10^-4 mol/L equol for 24,48 and 72h. After pretreatment with 10 μmol/L U0126 an ERK signaling pathway inhibitor or ICI182,780,estrogen receptor inhibitors for 1h,then treatment with 50 μmol/L equol for 2h,the cell viability was examined and the expressions of ERK and p-ERK protein were determined using Western blotting. Results Equol was demonstrated to inhibit SKOV-3,proliferation time-and dose-dependently. The expression of p-ERK protein was decreased in dose-dependent manner,while the expression of total ERK was unchanged. Both the single use of U0126,or ICI182,780,and combined with equol could decrease the expression of p-ERK protein. Conclusion Equol could inhibit proliferation in ovarian carcinoma cell lines SKOV-3. Its inhibitory effect appears to be due to down-regulation of p-ERK protein.

关 键 词：雌马酚 卵巢癌 ERK通路 

分 类 号：R737.31[医药卫生—肿瘤]