黄芪双向性固体发酵过程中黄芪甲苷的转化研究  

Transformation of Astragaloside IV in Bidirectional Solid Fermenting of Astragalus membranaceus

作　　者：阮鸣[1] 张李阳[1] 喻斌[2] 陈玉胜[1] 庄毅[3,4] 

机构地区：[1]南京晓庄学院生命科学系药用菌物研究所,江苏南京211171 [2]南京中医药大学江苏省中药药效与安全性评价重点实验室,江苏南京210029 [3]中国菌物学会 [4]南京中医药大学药用真菌研究中心,江苏南京210029

出　　处：《中药材》2010年第3期339-343,共5页Journal of Chinese Medicinal Materials

基　　金：江苏省教育厅项目(JH07-17);南京市科技局项目(2008创基030);南京晓庄学院重点研究项目(20007NXY16)

摘　　要：目的:研究黄芪双向性固体发酵前后黄芪甲苷的转化。方法:采用HPLC测定法、柱色谱分离法、波谱鉴定技术进行发酵前后的成分变化研究;观察转化产物对血管内皮细胞的抗氧化效应。结果:在黄芪发酵过程中,黄芪甲苷转化形成6-O-β-D-葡萄糖基-环黄芪醇,该转化物具有显著的抗氧化效应。结论:黄芪中有效成分黄芪甲苷经药用真菌(灵芝)双向性固体发酵后发生了生物转化,这一转化具有增效的作用。Objective：To study the transformation of Astragaloside IV in bidirectional solid fermenting of Astragalus membranaceus var.mongholicus.Methods：The chemical components of pre and post-fermenting were comparatively analyzed by techniques of HPLC,column chromatography and spectral identification.Antioxidant effect of transformational product on vascular endothelial cells was researched.Results：In fermenting of Astragalus membranaceus,Astragaloside IV was transformated into 6-O-β-D-glucoside cycloastragenol,which had significant antioxidant effect.Conclusion：Biotransformation of Astragaloside IV in Astragalus membranaceus was occurred after bidirectional solid fermenting with Ganoderma lucidum,and the biotransformation could enhance effect.

关 键 词：灵芝 黄芪 黄芪甲苷 双向性固体发酵 6-O-β-D-葡萄糖基-环黄芪醇 

分 类 号：R282.7[医药卫生—中药学] R284[医药卫生—中医学]