基质金属蛋白酶在早期肺癌中的表达  

The expression of matrix metalloproteinases in early stage no-small-cell lung cancer

作　　者：蔡铭[1] 翁鸢[1] 王志强[1] 朱扣军[1] 游庆军[1] 

机构地区：[1]苏州大学第四附属医院胸心外科,江苏无锡214062

出　　处：《中华实验外科杂志》2010年第5期612-613,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨早期肺癌中的基质金属蛋白酶（MMP）-9、MMP-2、MMP-7活性及与MMP抑制剂开发的关系。方法采用明胶酶谱及酪素酶谱法测定60例Ⅰ期非小细胞肺癌（NSCLC）患者的肺肿瘤及对应正常肺组织中MMP-9、MMP-2、MMP-7的活性。结果MMP-9活性均见于正常及癌组织中，但早期肺癌组织中的MMP-9的活性为（1189．3±537．0）灰度值，低于正常对照肺组织中（1557．7±422．5）灰度值（P〈0．05）。肿瘤的MMP-2的活性率（62kDa／62kDa＋66kDa）（45．5±8．4）％明显高丁相对应的正常组织中的（25．9±10．5）％（P〈0．01）。阳性对照的人羊水标本在酪素酶谱凝胶上显示MMP-7约20kI）a的透明活性带，但同时肺癌及正常肺组织标本未显示出MMP-7的可检测的活性。结论早期肺癌组织中MMP-9活性低及无明显MMP-7活性，MMP-2活性显著增高。Objective To investigate the activity of matrix metalloproteinases （MMP）-9, -2, -7 in non-small cell lung cancer （NSCLC） and the interrelationship between the expression of MMP and MMP inhibitor （MMPI） development. Methods The activity of MMP-2, MMP-9 and MMP-7 in 60 cases of stage Ⅰ NSCLC （36 cases of stage Ia, 24 cases of Ib 24 ; 25 cases of squamous carcinoma, 35 cases of adenocarcinoma） was measured by using gelatin zymography or casein zymography. Results The MMP-9 activity could be checked in hmg cancer tissue and normal tissue. However, in early stage NSCLC the average activity of MMP-9 （ 1189.3 ±537.0 pixel） was lower than in control normal lung tissue （ 1557.7 ±422. 5 pixel）, P 〈 0. 05. The MMP-2 activity rate in tumor （62 kDa/62 kDa ＋ 66 kDa） （45.5 ±8.4 ）% was significantly higher than that in normal lung cancer tissue （25.9 ± 10. 5）% （P 〈0. 01 ）. On casein zymography gel, the active MMP-7 in amniotic fluid showed a lucid band as 20 kDa, but no any detectable MMP-7 activity was found in lung cancer tissue and normal tissue. Conclusion In early stage of NSCLC, MMP-9 activity was low, there was no obvious MMP-7 activity, and the MMP-2 activity was significantly increased.

关 键 词：基质金属蛋白酶抑制剂 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]