反义pEGFP-C1-Survivin对骨肉瘤细胞生物学行为的影响  

The inhibitory effects of an antisense survivin vector on proliferation and invasion of osteosarcoma cells

作　　者：梁新军[1] 刘杨铭[2] 吴燕峰[1] 郭宇[1] 沈慧勇[1] 

机构地区：[1]中山大学附属第二医院骨科,广州510120 [2]湖北省疾病预防控制中心

出　　处：《中华实验外科杂志》2010年第5期637-639,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察Survivin反义核酸载体对人骨肉瘤细胞株MG-63生物学行为的抑制效应，并探讨其机制。方法MTT检测转染前后MG-63细胞增殖抑制率的变化，Boyden小室体外侵袭实验测定转染前后侵袭能力变化。逆转录-聚合酶链反应（RT-PCR）检测转染前后Ang-2基因mRNA表达的变化。结果噻唑蓝（MTT）检测显示转染后骨肉瘤细胞增殖明显受抑制，转染组抑制率达到23．4％，与各对照组之间差异有统计学意义（P〈0．01），脂质体组、空质粒组和空白组之间差异无统计学意义（P〉0．05）。RT-PCR分析显示转染组MG-63中Ang-2基因mRNA的表达较转染前明显下降，与其他组之间差异有统计学意义（P〈0．01），而另3组之间差异无统计学意义（P〉0．05）。Boyden小室体外侵袭实验测定显示转染组侵袭百分数为17．9％，与其他各组间差异有统计学意义（P〈0．01）。结论Survivin反义核酸可以显著抑制人骨肉瘤细胞株MG-63的增殖、侵袭能力，部分逆转其恶性表型，这可能与其下调Ang-2的表达有关。Objective To observe the inhibitory effects of an antisense Survivin vector on proliferation and invasion of osteosarcoma cells line MG-63, and explore the mechanism. Methods MTI＇ assay was used to measure the proliferation inhibition after transfection of MG-63 cells with Survivin antisense RNA. Boyden chamber in vitro invasion assay was applied to observe the changes of invasion capability. The expression of Ang-2 before and after transfection was detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results The proliferation of cells transfected with antisense Survivin was significant- ly inhibited （ the inhibition rate was 23.4% ） as compared with other groups. The invasion ability was remarkably decreased after antisense transfection （ 17.9% ） as compared with the control groups. Moreover, the mRNA expression of Ang-2 in antisense Survivin transfeeted cells was much lower than control groups. Conclusion The antisense Survivin vector could markedly inhibit the proliferation and invasion of osteosarcoma cells line MG-63, and partially reversed their malignant phenotype, which was possibly related with down-regulation of the Ang-2 expression.

关 键 词：骨肉瘤:Survivin ANG-2 

分 类 号：R738[医药卫生—肿瘤]