酒石酸锑钾对人结肠癌裸鼠移植瘤增殖细胞核抗原的影响  

The effect of PAT on PCNA of human colon carcinoma implants in nude mice

作　　者：杨艳果[1] 徐少勇[1] 张少君[1] 杜勇[1] 胡俊华[1] 

机构地区：[1]郧阳医学院附属人民医院消化内科,湖北省十堰442000

出　　处：《中国医师杂志》2010年第4期453-455,共3页Journal of Chinese Physician

基　　金：湖北省教育厅基金资助项目（2000A43003）

摘　　要：目的 探讨酒石酸锑钾（potassium antimony tartrate,PAT）对人结肠癌移植瘤裸鼠模型抑瘤及凋亡诱导作用.方法 60只裸鼠皮下注射结肠癌细胞株SW480建立移植瘤模型,随机分为4组（n=15）.分别为生理盐水组、5-氟尿嘧啶（5-Fu）50 mg/（Kg·d）组、酒石酸锑钾组[（20 mg/（Kg·d）和40 mg/（Kg·d）],给药15 d,每3日测量肿瘤大小1次,绘制肿瘤生长曲线;末次给药24 h后,免疫组织化学法检测结肠癌细胞增殖细胞核抗原（PCNA）的表达.结果 使用PAT后,肿瘤体积增长较慢,PAT处理组细胞PCNA表达[（63.63±8.88）%,（59.13±6.15）%,（33.38±12.76）%]较对照组[（69.88±8.81）%]明显下调（P〈0.05或P〈0.01）.结论 PAT能有效抑制结肠癌细胞生长和诱导结肠癌细胞凋亡.Objective To study the potential therapeutic effect of potassium antimonyl tartrate （PAT） on human colon cancer in transplanted tumor nude mice models. Methods Sixty transplanted animal models were constructed with colon cancer cell line SW480 injected in nude mice. Nude mice were then random divided into 4 groups （ n = 15） ：Normal saline group, 5-Fu group and different dose of PAT groups [ （20 mg/（ Kg · d） ,40 mg/（ Kg · d） ]. The volume of mass was measured every 3 days. After final-administration for 24 hours, immunohistochemical staining was used to detect the expression of PCNA in colon cancer cells. Results After the use of PAT, the growth of mass slowed down. PCNA levels [ （63. 63 ±8. 88）% ,（59. 13 ±6. 15）% ,（33. 38 ± 12. 76）% ] in SW480 cells was reduced by PAT（ P 〈0. 05, P 〈0. 01 ）. Conclusion PAT potentially inhibited the growth of colon cell lines and induced apoptosis of SW480 colon cancer cells.

关 键 词：酒石酸锑钾/药理学 结肠肿瘤/药物疗法 增殖细胞核抗原/药物作用/代谢 

分 类 号：R735.35[医药卫生—肿瘤]