CD40蛋白质在膀胱尿路上皮癌组织中的表达及其临床意义  

Expression and clinical significance of CD40 protein in human bladder cancer tissue

作　　者：阳宁[1] 罗志刚[1] 

机构地区：[1]南华大学第二附属医院泌尿外科,湖南衡阳421001

出　　处：《肿瘤研究与临床》2010年第4期219-221,共3页Cancer Research and Clinic

摘　　要：目的研究CD40在膀胱尿路上皮癌的表达，探讨其与肿瘤分期、病理分级、细胞凋亡之间的相关性。方法采用免疫组织化学技术检测20例膀胱癌旁组织及78例膀胱尿路上皮癌组织中CD。的表达状况，Hoechst法检测膀胱尿路上皮癌组织细胞凋亡情况，确定凋亡率。结果CD。在20例膀胱癌旁组织中2例表达（10％），在78例膀胱尿路上皮癌组织中55例表达（70．5％），差异有统计学意义（_P〈0．01），CD40的表达与膀胱尿路上皮癌的临床分期、病理分级呈负相关。CD40阳性组细胞凋亡率为（12．60±0．38）％，阴性组细胞凋亡率为（6．77±0．53）％，差异有统计学意义（P〈0．01），提示CD40可能诱导细胞凋亡。结论CD40在膀胱尿路上皮癌组织的表达与肿瘤的临床分期、病理分级、细胞凋亡密切相关，可为膀胱尿路上皮癌的诊断、治疗及指导预后提供实验依据。Objective To investigate the expression of CD40 protein in bladder urothelium cancer and to explore its correlation with clinical stage, pathologica] grades, cell apoptosis. Methods Expression of CD40 protein was evaluated by immunohistochemistry in paracancerous tissues （20 cases） and bladder urothelium cancer tissues （78 cases）. Apoptosis of bladder urothelium cancer tissues was detected by Hoeehst to determine the apoptotie rate （AR）. Results The expression rate of CD40 protein was 2/20（10 %） in the cases of paracaneerous tissues, 55/78（70.5%） in the cases of bladder urothelium cancer tissues, a statistically significant differences of CD40 staining was observed between paraeancerous tissues and bladder urothelium cancer tissues （P 〈0.01）. Overexpression of CD40 was strongly inversely related to the tumor grade and clinical stage; the positive group of CD40 protein was （12.60±0.38）%, the negative group of CD40 protein was （6.77± 0.53）%, a statistically significant differences of apoptosis were observed between CD40 protein positive group and CD40 protein negative group （P 〈0.01）. It showed CD40 protein might induce cell apoptosis. Conclusion Expression of CD40 protein is closely related with clinical stage, pathological grade and cell apoptotic rate and may offer reliable evidence for diagnosis, treatment and prognosis in bladder urothelium cancer.

关 键 词：膀胱尿路上皮癌 CD40蛋白质 细胞凋亡 

分 类 号：R737.14[医药卫生—肿瘤]