胃癌中P-糖蛋白、多药耐药相关蛋白、肺耐药蛋白的表达及其意义  

Expression and significance of P-gp, LRP, MRP in gastric cancer

作　　者：胡文庆[1] 闫曙光[1] 王泽华[1] 彭春伟[2] 周英[1] 李雁[2] 

机构地区：[1]长治医学院附属和济医院普外科,046011 [2]武汉大学中南医院肿瘤科肿瘤生物学行为湖北省重点实验室

出　　处：《肿瘤研究与临床》2010年第4期247-249,254,共4页Cancer Research and Clinic

基　　金：基金项目：山西高校科技研究开发项目;教育部新世纪优秀人才支持计划（NCET一04-0669）

摘　　要：目的检测胃癌组织中P一糖蛋白（P—gp）、多药耐药相关蛋白（MRP）、肺耐药蛋白（LRP）的表达，分析其与胃癌临床病理因素的关系。方法采用免疫组织化学SP法对59例胃癌患者癌组织进行研究。结果在59例胃癌患者中P-gp阳性率最高[86．4％（51例）]，其次为LRP[84．7％（50例）]，二者呈正相关（r=0．803）；MRP阳性率最低[27．1％（16例）]。P-gp、MRP、LRP与分化程度无关（均P〉0．05）。P-gp与临床分期呈正相关（r=0．742），MRP、LRP均与胃癌浸润深度、淋巴结转移与否及分化程度无关。结论P-gp、MRP、LRP在未接受化疗胃癌组织中高表达，提示胃癌中存在P-gp、MRP、LRP介导的原发性耐药。Objective To detect the expression of muhidrug resistance proteins P-gp, LRP, MRP, and analyze the relationship between them and the clinicopathological factors. Methods The expression of P-gp, LRP, MRP in formalin-fixed paraffin-embedded tissue sections was determined by a labelled Streptavidin- Peroxidase （SP） immunohistochemical technique, and the results were analyzed in correlation with clinicopathological data. None of these patients received chemotherapy prior to surgery. Results The positive rates of P-gp, LRP, MRP were 86.4 %, 84.7 % and 27.1%, respectively. The difference between the positive rate of P-gp and MRP was significant statistically, as well as the difference between the expression of MRP and LRP. No significant difference was observed between P-gp and LRP, but the positively correlation between the expression of P-gp and LRP was observed. No significant correlation between the expression of P-gp, LRP, MRP and the grade of differentiation were observed. The expression of P-gp was correlated with clinical stages positively （r=0.742）, but the difference with the expression of P-gp in different stages was not significant. Conclusion The expression of P-gp, LRP, MRP in patients with gastric cancer without prior chemotherapy are high and indicates that innate drug resistance may exist in gastric cancer.

关 键 词：胃肿瘤 P-糖蛋白 多药耐药相关蛋白类 肺耐药蛋白 辅助化疗 

分 类 号：R735.2[医药卫生—肿瘤]