鸟氨酸脱羧酶抗酶抑制子小鼠体内的表达分析  

Analysis of the expression of ornithine decarboxylase antizyme inhibitor in mouse tissues

作　　者：王增产[1] 张小花[1] 万涛[1] 王李英[1] 刘湘[1] 汤华[1] 

机构地区：[1]重庆医科大学感染性疾病分子生物学重点实验室,重庆400016

出　　处：《重庆医科大学学报》2010年第3期332-334,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金(编号:30671080);重庆市科委项目(编号:CSTC;2008BB5237)

摘　　要：目的:分析鸟氨酸脱羧酶抗酶抑制子(Ornithine decarboxylase antizyme inhibitor,AZI)在小鼠体内的表达情况。方法:RT-PCR,Northern blot,免疫组化染色。结果:RT-PCR,Northern blot揭示AZT基因(Oazin)的表达是全身性的,即各器官都表达。特别是脑,心,肾脏,肌肉,胸腺,睾丸。免疫组化染色显示AZT蛋白在心瓣膜细胞,肾小管周围的间质细胞以及肝细胞的间质细胞中高表达。结论:用RT-PCR,Northern blot以及免疫组化染色法揭示了AZT在RNA和蛋白水平小鼠体内中的表达情况。Objective：Analysis of the expression of Ornithine decarboxylase antizyme inhibitor in mouse tissues.Methods：RT-PCR,Northern blot,immunostaining.Results：RT-PCR,Northern blot reveal that the expression of Ornithine decarboxylase antizyme inhibitor gene（AZI） is ubiquitous,especially high in brain,heart,kidney,muscle,thymus and testis.Immunostaining reveals that AZI expresses on valve cells in heart,mesenchyme cells in kidney and liver.Conclusion：The expression of AZI in mouse tissues was identified by using RT-PCR,Northern blot and immunostaining.

关 键 词：鸟氨酸脱羧酶抗酶抑制子 表达 

分 类 号：R383.24[医药卫生—医学寄生虫学]