兔VX2乳腺癌CT灌注成像与血管内皮生长因子表达的相关性研究  

Correlative investigation of functional multi-slice computed tomography perfusion imaging with the expression of vascular endothelial growth factor on rabbit model with VX2 breast cancer

作　　者：雷振[1] 徐娜[1] 伍建林[2] 席焕久[3] 

机构地区：[1]辽宁医学院附属第一医院放射科,锦州121001 [2]大连医科大学附属第一医院放射科 [3]辽宁医学院附属第一医院人类学研究所,锦州121001

出　　处：《中华放射学杂志》2010年第5期527-530,共4页Chinese Journal of Radiology

基　　金：辽宁省自然基金（20062162）

摘　　要：目的 对兔VX2乳腺癌模型功能MSCT（fMSCT）灌注参数与免疫组织化学（简称免疫组化）检测的血管内皮生长因子（VEGF）进行相关性分析,在体无创评价未经治疗的兔VX2乳腺癌血管生成特点.方法 纯种雌性新西兰大白兔16只,于兔乳晕周围注入VX2组织块悬液1 ml.瘤组织生长2周后对荷瘤兔进行CT灌注扫描,获得血流量（BF）、血容量（BV）、平均通过时间（MTT）及表面通透性（PS）灌注参数均值,16只荷瘤兔取瘤组织进行免疫组化检测VEGF表达.对获得的肿瘤与肌肉灌注参数值进行两样本t检验,灌注参数与VEGF值间用Pearson相关性分析.结果 兔VX2乳腺癌肿瘤CT灌注参数BF、BV、MTT和PS均值分别为（228.21±13.13）ml·min-1·100 mg-1、（13.45±1.01）ml·100 mg-1、（3.50±0.20）s、（7.85±1.18）ml·min-1·100 mg-1;兔肌肉组织CT灌注参数BF、BV、MTT和PS均值分别为（66.10±22.11）ml·min-1·100 mg-1、（1.88±1. 80）ml·100 mg-1、（23.87±0.63）s、（1.55±0.38）ml·min-1·100 mg-1,肿瘤CT灌注参数BF、BV和PS均值明显高于肌肉组织,MTT低于肌肉组织,2组间的BF、BV、MTT及PS差异具有统计学意义（t值分别为61.83、13.63、27.72、20.54,P值均＜0.01）.兔VX2乳腺癌组织VEGF表达均值为7.33±0.27,分别与BF（r=0.712,P＜0.01）、BV（r=0.647,P＜0.01）和PS（r=0.627,P＜0.01）存在正相关,与MTT（r=-0.564,P＜0.05）存在负相关.结论 MSCT灌注成像可以在体无创评价未经治疗的兔VX2乳腺癌血管生成.Objective To analyze the correlation of perfusion parameters obtained by functional multi-slice computed tomography （fMSCT） with vascular endothelial growth factor （VEGF） detected with immunohistochemistry on rabbit model with VX2 breast cancer and noninvasively evaluate the vascularization of untreated VX2 breast cancer in vivo. Methods Sixteen New Zealand femal rabbits were selected and suspension （1 ml） of mass was injected around the breast areola. CT perfusion was performed after two weeks and perfusion parameters including blood flow （BF）, blood volume （BV）, mean transit time （MTT）and permeability surface （PS） were assessed. Expression of VEGF in neoplasm was detected with immunohistochemistry. Paried t test was used for the comparison of perfusion parameters between the tumor and muscle and Pearson correlation analysis was used to assess the correlation of VEGF with perfusion parameters. Results The mean value of BF, BV, MTT and PS were （228.21 ± 13. 13 ） ml · min-1100 mg-1 ,（13.45 ± 1.01） ml · 100 mg-1 ,（3. 50 ±0. 20） sand （7.85 ±1.18） ml · min-1 · 100 mg-1 in tumor, respectively. They were （66. 10 ±22. 11 ） ml · min-1 · 100 mg-1 , （ 1.88 ± 1.80） ml · 100 mg-1,（23. 87 ±0. 63）s,（1.55 ±0. 38）ml · min-1 · 100 mg-1 in muscular tissue, respectively. The mean value of BF, BV and PS in tumor were obviously higher than those in muscle, and the mean value of MTT in tumor was lower than that in muscle. There were significant differences in CT perfusion parameters between tumor and muscle （t = 61.83,13.63,27.72,20. 54, P 〈 0. 01 ）. The mean value of VEGF in tumor was 7. 33 ±0. 27 and there were positive correlation with BF （ r = 0. 712, P 〈 0. 01 ）, BV （ r = 0. 647, P 〈 0. 01 ）, PS （ r =0. 627 ,P 〈 0. 01 ）, and negative correlation with MTT （ r = - 0. 564, P 〈 0. 05 ）. Conclusion MSCT perfusion imaging can be used to noninvasively evaluate the vascularization in rabbits with untreated VX2 breast cancer in vivo

关 键 词：乳腺肿瘤 内皮生长因子 体层摄影术 X线计算机 动物实验 

分 类 号：R737.9[医药卫生—肿瘤]