缺氧诱导因子-1α与EphA2在食管鳞状细胞癌中的表达及其相关性研究  

Expression and correlation of hypoxia-inducible factor-1α and EphA2 in esophageal squamous cell carcinoma

作　　者：金海林[1] 施瑞华[1] 朱宏[1] 张伟锋[1] 曾楷峰[1] 都波[1] 

机构地区：[1]南京医科大学第一附属医院消化科,南京210029

出　　处：《中华消化杂志》2010年第3期184-188,共5页Chinese Journal of Digestion

基　　金：基金项目：国家自然科学基金资助项目（30770991）

摘　　要：目的检测缺氧诱导因子-1α（HIF-1α）蛋白与EphA2蛋白在食管鳞状细胞癌中的表达定位并探讨二者表达的相关性。方法采用细胞免疫荧光法检测HIF1a与EphA2在食管鳞状细胞癌细胞株Eca109中的表达与定位；采用免疫组化法检测HIF-1α与EphA2在41例食管鳞状细胞癌和25例正常食管组织中的表达；常氧和缺氧条件下，Western印迹法检测食管鳞状细胞癌细胞株Eca109和TE13经RNA干扰（RNAi）技术特异性沉默HIF-10后EphA2表达的变化。结果①细胞免疫荧光结果显示HIF-1α和EphA2均在Eca109细胞胞质表达。②免疫组化结果显示HIF-la在食管鳞状细胞癌和正常食管组织中的阳性表达率分别为70．73％（29／41）和0％（0／25），两者比较差异有统计学意义（P〈O．05）。EphA2在食管鳞状细胞癌和正常食管组织中的阳性表达率分别为78．04％（32／41）和28％（7／25），两者比较差异有统计学意义（P％0．05）。相关性检验提示HIF-1d和EphA2在食管鳞状细胞癌中表达呈正相关性（r-0．5654，x2=13．11，P〈0．05）。③Western印迹结果显示在食管鳞状细胞癌细胞株Ecal09和TEl3中，EphA2表达随HIF-1α的沉默而受抑制。结论HIF-1α与EphA2在食管鳞状细胞癌组织中呈高表达，且二者表达呈正相关；EphA2表达受HIF-1α的调控，可能为HIF-1α的靶基因。Objective To investigate the expressions and the correlation of hypoxia-inducible （actor（HIF）-1α and EphA2 in esophageal squamous cell carcinoma. Methods The subcellular localizations of HIF-1α and EphA2 in Ecal09 cell line were characterized by immunofluorescence staining. The expressions of HIF-la and EphA2 in human esophageal squamous cell carcinoma tissues were detected by immunohistochemistry. Western blot was used to detect the expression of EphA2 in Eeal09 cell line with knock-down HIF-1α gene and in TE13 cell line under normoxia and hypoxia in vitro. Results （1） HIF-1α and EphA2 could be detected in cytoplasm in Ecal09. （2） The positive rates of HIF-1α and EphA2 were significantly higher in esophageal sqnamous cell carcinoma tissues than those in normal esophagus tissues （70.73% vs 0%, P〈0.05; 78.04% vs 28%, P〈0.05）. The correlation analysis xindicated that the expression of EphA2 had a positive correlation with that of HIF- la in esophageal squamous cell carcinoma. （3） Western blot showed that the expression of EphA2 in Ecal09 and TEl3 was inhibited with silencing HIF-1α. Conclusions HIF-1α and EphA2 are overexpressed in esophageal squamous cell carcinoma tissues. The expression of EphA2 protein positively correlates with expression of HIF-1α protein. EphA2 may be a potential target of HIF -1α

关 键 词：食管肿瘤 缺氧诱导因子-1 EPHA2 RNA干扰 转染 

分 类 号：R735.1[医药卫生—肿瘤]