β-榄香烯对胃癌及胃癌耐药细胞杀伤作用的实验研究  

Cytotoxieity effect of β-elemene on gastric cancer and drug resistant cells

作　　者：王晓瑾[1] 赵晓莹[1] 诸琦[1] 章永平[1] 黄佳[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院消化科,200025

出　　处：《中华消化杂志》2010年第3期189-192,共4页Chinese Journal of Digestion

摘　　要：目的研究β-榄香烯联合或不联合化学治疗药物5-氟尿嘧啶（5-FU）对人胃癌细胞株SGC-7901和相应耐药细胞株SGC-7901／5-FU的杀伤作用及机制。方法用不同剂量β-榄香烯（20、40或80ug／m1）联合或不联合5-FU（100μg／m1）作用于SGC-7901和SGC-7901／5Fu细胞，采用四甲基偶氮唑盐实验、透射电镜观察、流式细胞仪和DNA原位末端标记（TUNEL）法检测药物对细胞的杀伤作用及其诱导细胞凋亡的情况。通过建立SGC-7901和SGC-7901／5-Fu细胞裸鼠移植瘤模型观察p-榄香烯对这两种细胞的体内杀伤作用。结果β-榄香烯在体内、外均具有抑制SGC-7901和SGC-7901／5-FU细胞生长的作用（P值均d0．05），-定剂量范围内具量效关系（P=0．02）。透射电镜、流式细胞仪和TUNEL实验均显示β-榄香烯抑制两种胃癌细胞生长的作用与其诱导细胞凋亡有关。结论β-榄香烯在体内外对SGC7901和SGC-7901／5-FU细胞均具杀伤作用，该作用可能与其诱导细胞凋亡有关。Objective To investigate the cytotoxicity of β-elemene combined with or without 5- FU on gastric cancer cell line SGC 7901 and drug resistant cell SGC-7901/5-FU as well as its potential mechanism. Methods SGC-7901 and SGC-7901/5-FU were treated with β-etemene combined with or without 5-FU （0 or 100 μg/ml） in different doses （ 20,40 or 80μg/ml）. The cytotoxicity of β-elemene on SGC-7901 and SGC-7901/5 FU were analyzed by MTT, and apoptosis of the cells induced by β- elemene was detected by transmission electron microscope, flow cytometry and TUNEL. Eighty xenograft models were established in athymic mice and the cytotoxicity of β-elemene on SGC-7901 and SGC7901/5-FU in vivo was assessed by calculating tumor suppressing rate. Results The inhibitory effect of β-elemene on both SGC-7901 and SGC- 7901/5-FU was found in a close dependent manner （P〈0.05） in vitro and in vivo. The examination showed that the cytotoxicity of β-elemene on both SGC-7901 and SGC- 7901/5-FU was related to its induction of apoptosis. Conclusions The cytotoxicity of β- elemene on gastric cancer and its drug resistant cells was proved in vitro and in vivo, which may be acted by inducing apoptosis of cells.

关 键 词：胃肿癌 榄香烯 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]