鼻咽癌细胞系候选癌基因的筛选  

Screening of the candidate oncogenes in nasopharyngeal carcinoma cell lines

作　　者：樊英[1] 李佳[1] 黄仲曦[1] 姚开泰[1] 

机构地区：[1]南方医科大学肿瘤研究所,广州市510515

出　　处：《实用医学杂志》2010年第9期1502-1504,共3页The Journal of Practical Medicine

摘　　要：目的:利用SNP芯片和基因表达谱芯片数据筛选鼻咽癌细胞系CNE1、CNE2、HONE1中的候选癌基因。方法:SNP芯片检测DNA拷贝数,基因表达谱芯片检测3个鼻咽癌细胞系与人永生化鼻咽上皮细胞系NP69基因表达水平,Ensembl database将SNP数据、基因表达谱数据和人类基因组中已知的DNA拷贝数改变数据整合,筛选候选癌基因,生物信息学进一步分析功能。结果:筛选出候选癌基因102个,包含许多功能群,参与各种信号通路。结论:为癌基因的筛选提供了一种研究方法;筛选鼻咽癌细胞系中候选癌基因,对其的进一步研究有助于认识鼻咽癌的发病机制,为鼻咽癌的诊断和治疗提供理论基础。Objective To screen the candidate oncogenes in nasopharyngeal carcinoma （NPC） cell lines CNE1, CNE2, and HONE1. Methods Detected DNA copy number （DCN） by 250k single nucleotide polymorphism （SNP） arrays, used Affymetrix human genome U133 plus 2.0 microarrays to measure gene expression levels of NPC cell lines and immortalized nasopharyngeal epithelial cell line NP69. Then, DCN data, DNA copy number variation data and gene expression data were integrated by the Ensembl database. At last, screened the candidate oncogenes from the integrated database, further analyzed them with bioinformatics. Conclusions We provide a method for screening oncogenes. Screening of candidate oncogenes in NPC cell lines and further analysis of the obtained genes will be helpful to illuminating the pathogenesis of NPC, and offering rationale for diagnosis and treatment of NPC.

关 键 词：鼻咽肿瘤 SNP芯片 基因表达谱芯片 候选癌基因 

分 类 号：R739.63[医药卫生—肿瘤]