气候因素对狍种群遗传多样性的影响  

Effects of ecological factors on the population genetic diversity in roe deer (Capreolus Pygargus)

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作  者:刘艳华[1] 贾竟波[1] 张明海[1] 

机构地区:[1]东北林业大学

出  处:《生态科学》2010年第1期70-74,共5页Ecological Science

基  金:黑龙江省自然科学基金重点资助项目(ZJN-0501);国家“十一五”科技支撑计划重点课题(2008BADB0B06);美国老虎犀牛保护基金(98210-2-G191)资助

摘  要:采用聚合酶链式反应(PCR)技术分析测定了6个狍(Capreolus Pygargus)种群的分子遗传特征。遗传分析表明:狍迎春种群具有较低的单倍型多样性(H=0.622±0.138)和核苷酸多样性(π=0.386±0.00383),图强种群具有较高的单倍型多样性(H=0.857±0.044)和核苷酸多样(π=2.580±0.01914),Taiima’sD和FuandLi’sD值检测结果表明这6个狍种群相对于中性进化的歧异度并没有明显的偏离(P〉0.1):相关性分析表明:狍遗传多样性与纬度(r=0.770)和海拔(r=0.719)呈著正相关,与年平均气温(r=-0.519)和无霜期(r=-0.652)呈显著负相关,与经度(r=-0.258)和年平均降水量(r=.0.205)呈显著的不相关。We used polymerase chain react ion (PCR) to analyze mtDNA control region gene of 65 individuals from six populations. The results showed that there were lower haplotype diversity (H=0.622±0.138) and nucleotide diversity (π=0.386±0.00383) in Yingchun population, higher haplotype diversity (H=0.857±0.044) and nucleotide diversity (π=2.580±0.01914) in Tuqiang; Neither the estimate of Tajima's D nor that of Fu and Li's D deviated significantly from the neutral selection hypothesis (P 〉 0. 1) for six populations, showing no evidence of strong selective sweeps or balancing selection. Correlation analysis shows the genetic diversity was significant positive correlation with latitude (r= 0.770) and altitude (r= 0.719), significant negative correlation with annual average temperature (r= - 0.519) and frostless days (r= - 0.652), no significant correlation with longitude (r= - 0.258) and annual precipitations (r= - 0.205).

关 键 词:狍(Capreolus Pygargus) 粪便DNA 遗传多样性 生态因子 

分 类 号:Q141[生物学—生态学]

 

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