急性坏死性胰腺炎大鼠腹腔内压力和血TNF-α的时相改变  

Time-course changes of intra-abdominal pressure and plasma TNF-α in rats with acute necrotizing pancreatitis

作　　者：贾林[1] 陈奕金[1] 黄耀星[1] 邱嘉华[1] 杜洪[2] 

机构地区：[1]广州医学院附属广州市第一人民医院广州市南沙中心医院消化内科,广州510180 [2]广州医学院附属广州市第一人民医院广州市南沙中心医院病理科,广州510180

出　　处：《中华胰腺病杂志》2010年第2期106-108,共3页Chinese Journal of Pancreatology

基　　金：广州市卫生局重点专科基金（2008-ZDi-02）

摘　　要：目的 探讨急性坏死性胰腺炎（ANP）大鼠模型的腹腔内压力和TNF-α的时相改变及其规律.方法 80只SD大鼠按数字表法分为ANP组和对照组.采用4.5%牛磺胆酸钠胰管内逆行注射制备ANP模型,对照组注射等量生理盐水.制模后1、3、6、12、24 h分批各处死8只大鼠,检测血淀粉酶、TNF-α水平,测定腹水量和腹腔内压力,并观察胰腺病理改变.结果 ANP大鼠血淀粉酶含量呈进行性升高,24 h时高达对照组的32倍;血TNF-α水平也显著升高,6 h时达到峰值,为对照组的18倍;制模后1 h起腹水量开始增多,于24 h达到高峰,约为对照组4.7倍;制模后1 h起腹内压力明显升高,3 h时约为对照组的3倍,12 h约为对照组的9倍;胰腺病理损伤进行性加重,24 h损伤最严重.结论 ANP大鼠存在明显腹内高压,制模后12 h腹内压最高,炎症介质TNF-α升高高峰为制模后6 h,故腹内高压可能为炎症反应的后续反应.Objective To investigate the time-course change of intra-abdominal pressure （IAP）, TNF-α in rat with acute necrotizing pancreatitis （ANP） and its mechanism. Methods 80 SD rats were randomly divided into ANP group and control group. ANP group was induced by retrograde injection of 4.5% sodium taurocholate into the pancreatic duct in SD rats, while control group was induced by injection of saline at the same dosage. Eight rats in each group were killed at 1 h, 3 h, 6 h, 12 h and 24 h, respectively. The serum levels of amylase, TNF-α were determined;IAP, amount of abdominal ascites, pancreatic pathologic changes were evaluated. Results The levels of amylase in ANP group increased progressively with time, which were 32-folds higher than those in control group at 24 h. The serum levels of TNF-α in ANP group reached the peak at 6 h, and were significantly higher than those in the control group. The amount of abdominal ascites increased post-ANP induction and reached the peak at 24 h, which was 4.7-folds higher than that in the control group. The IAP significantly increased 1 h after induction, which was 3 times as high as the control group in 3 h group and 9 times in 12 h group. Pathologic injuries deteriorated progressively and reached the peak at 24 h. Conclusions Significant IAP occurred in ANP rats and reached the peak at 12 h, TNF-α reached the peak at 6 h, therefore IAP may be the secondary response to inflammatory reaction.

关 键 词：胰腺炎 急性坏死性 压力 肿瘤坏死因子Α 大鼠 

分 类 号：R576[医药卫生—消化系统]