蝎毒多肽提取物对白血病小鼠Bcl-2 SDF-1α与TGF-β1表达的影响  

The Effect of PESV on Bcl-2,SDF-1α and TGF-β1 Expression in Leukemia in Mice

作　　者：杨文华[1] 杨向东[1] 史哲新[1] 高宏[1] 汤毅[1] 于文俊[2] 吕俊秀[2] 郝征[2] 

机构地区：[1]天津中医药大学第一附属医院血液科,天津市300193 [2]天津中医药大学

出　　处：《中国肿瘤临床》2010年第8期429-432,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:30672743);天津市应用基础研究项目基金资助(编号:07JCYBJC11500)~~

摘　　要：目的:探讨蝎毒多肽提取物(PESV)对白血病小鼠Bcl-2、SDF-1α与TGF-β1表达的影响,探究PESV对白血病细胞增殖和浸润的影响与机制。方法:NOD-SCID小鼠60只,按本课题前期研究方法,建立人白血病NOD-SCID小鼠髓外浸润模型。选取造模成功的小鼠40只,随机分为4组,每组10只,分别为高、中、低剂量组和模型组,另设同周龄正常NOD-SCID小鼠10只为空白对照组。高、中、低剂量组每只分别尾静脉注射PESV 1.2mg/(kg·d)、0.6mg/(kg·d)、0.3mg/(kg·d),模型组和空白组每只均尾静脉注射0.9%的生理盐水0.3mL/d,35d后全部处死,取血清和骨髓细胞培养液上清,用ELISA法进行Bcl-2、SDF-1α与TGF-β1的检测。结果:高、中、低剂量组存活率均高于模型组,高、中、低剂量组小鼠血清以及骨髓的Bcl-2、SDF-1α均明显低于模型组(P<0.05),而TGF-β1均高于模型组,均以高剂量组效果最为明显(P<0.05)。结论:蝎毒多肽提取物可以有效的降低白血病小鼠血清及骨髓中Bcl-2、SDF-1α的表达,提高TGF-β1的表达,具有较好的阻抑白血病细胞增殖和浸润的作用。Objective： To investigate the effect of PESV on the expression of Bcl-2, SDF-1α and TGF-β1 and the multiplication and infiltration of leukemic cells in leukemia in mice. Methods： A total of 60 NOD-SCID mice were used for model establishment of human leukemia with extra-marrow infiltration. Fourty successful models were randomly divided into 4 groups, with 10 in each group （high-dose group, medium-dose group, low-dose group and model group）. Another 10 normal NOD-SCID mice of the same age were used as the blank control group. In high-, medium- and low-dose groups, the mice were injected through tail vein with PESV at 1.2 mg/kg·d, 0.6 mg/kg ·d, 0.3 mg/kg·d, respectively. Mice in the model group and the blank control group received treatment of 0.9% physiological saline at 0.3 mL/d. The mice were sacrificed at 35 days after treatment. Elisa was used to detect Bcl-2, SDF-1α and TGF-β1 in cell culture supernatant of serum and bone marrow. Results： The survival of mice in high-, medium- and low-dose groups was higher than that in the model group. Bcl-2 and SDF-1α expression in serum and bone marrow of the mice in high-, medium- and low-dose groups was significantly lower than that in model group （P〈0.05）. TGF-β1 expression was higher in serum and bone marrow of the mice in high-, medium- and low-dose groups than in model group （P〈0.05）. Conclusion： PESV can effectively reduce the expression of Bcl-2 and SDF-le in serum and bone marrow of mice with leukemia and enhance TGF-β1 expression, and thus has inhibitory effect on multiplication and infiltration of leukemia cells.

关 键 词：蝎毒多肽提取物 白血病 BCL-2 SDF-1Α TGF-Β1 

分 类 号：R739.6[医药卫生—肿瘤]