人胰岛素样生长因子Ⅱ反义RNA对肝癌细胞恶性表型的抑制作用  

Anhibitory effect of IGF-II antisense RNA on malignant phenotype of hepatocellular carcinoma

作　　者：杨冬华[1] 张鸣青[1] 杜江[2] 徐重[2] 梁巧明[2] 毛积芳[3] 覃汉荣[1] 范子荣[1] 

机构地区：[1]第一军医大学珠江医院消化科,广州510282 [2]第一军医大学珠江医院分子生物学实验室,广州510282 [3]上海第二军医大学生物化学研究室

出　　处：《中华肝脏病杂志》1999年第1期39-41,共3页Chinese Journal of Hepatology

基　　金：广东省自然科学基金!NO.940319

摘　　要：研究人胰岛素样生长因子Ⅱ（ＩＧＦ－Ⅱ）反义ＲＮＡ对肝癌细胞株ＳＭＭＣ－７７２１的抑制效应。方法人ＩＧＦ－ⅡｃＤＮＡ０．１ｋｂ反向插人真核细胞表达载体ｐｃＮＡ３，获得ＩＧＦ－Ⅱ反义ＲＮＡ表达载体ｐＩＧＦ－ⅡＡＳ，将其导人人肝癌细胞株ＳＭＭＣ-７７２１，观察软琼脂培养集落形成的能力。流式细胞仪（ＦＣＭ）测定ｐＩＧＦ－ⅡＡｓ表达ＩＧＦ－Ⅱ反义ＲＮＡ对ＳＭＭＣ-７７２１细胞生长的影响。结果转导人ｐＩＧＦ－ⅡＡｓ的ＳＭＭＣ－７７２１细胞，不能在软琼脂上形成集落，ＦＣＭＭ实导人ＰＩＧＦ－ⅡＡｓ细胞Ｓ期增多，而对照组ＳＭＭＣ－７７２１细胞和带ＤＣＤＮＡ３空载体的ＳＭＭＣ－７７２１细胞则无明显变化．结论ｐＩＧＦ-ⅡＡＳ＆义ＲＮＡ体外可抑制肝癌细胞株ＳＭＭＣ－７７２１的致瘤性。Objetive Inhibitory effect of insulin like growth factor II (IGF- II ) antisense RNA onmalignant phenotype of hepatic cancer cells was studied. Methods A 0.lkb cDNA of human IGF- wasreversely inserted into a eukaryotic expression vector of pcDNA3 and an IGF antisense RNA expressionvector of PIGF- As was obtained. The pIGF- As was then introduced into human hepatic cancer cellline SMMC-7721. The effect of IGF- antisense RNA Which was expressed by pIGF- As on SMMC7721 cell cycles progression was measured with FCM. Results The coloning formation in dependent assay of SMMC-7721 cells with pIGF-As was significantly decreased by comparision withcontrol groups of SMMC-7721 Cells and SMMC-7721 cells transfected with pcDNA3 vector alone. FCManalysis showed that the s phase of cell cycles for SMMC-7721 cells with pIGF-As was increased, butthere were no obvious changes in the control groupe of SMMC-7721 cells and SMMC-7721 cells withpcDNA3. Conclusion The pIGF-As of IGF- antisense RNA acts as an inhibitor on carcinogenesis ofthe SMMC-7721 Cells in vitro.

关 键 词：肝细胞癌 胰岛素样 生长因子 肿瘤抑制基因 

分 类 号：R735.705[医药卫生—肿瘤] R730.51[医药卫生—临床医学]