重组人白介素-1α对人牙周膜成纤维细胞表达RANKL和OPG影响的荧光定量RT-PCR研究  

Effects of Recombinant Human Interleukin-1α on the Gene Expression of RANKL and OPG in HPDLFs by Using FQ-RT-PCR Method

作　　者：陈良娇[1] 兰泽栋[2] 刘嵘[3] 

机构地区：[1]福建医科大学口腔医学院正畸科,福建福州350004 [2]广州医学院口腔医院正畸科 [3]深圳市儿童医院口腔科

出　　处：《口腔医学研究》2010年第2期197-200,共4页Journal of Oral Science Research

摘　　要：目的:通过观察重组人白介素-1α(rhIL-1α)对体外培养人牙周膜成纤维细胞(HPDLFs)核因子kB受体活化因子配基(RANKL)和骨保护素(OPG)表达的影响来探讨牙槽骨改建的调节机制。方法:以不同浓度rhIL-1α(0、5、10、20μg/L)作用于体外培养HPDLFs,于24 h后收集细胞,利用荧光定量RT-PCR技术检测RANKLmRNA和OPG mRNA的表达。结果:rhIL-1α同时上调HPDLFs表达RANKL mRNA和OPG mRNA,但RANKL/OPG的比值增加,这种调节作用在10μg/L的作用浓度时最明显。结论:rhIL-1α可在体外影响HP-DLFs表达RANKL mRNA和OPG mRNA,调节RANKL/OPG比值,与牙槽骨改建密切相关。Objective： To investigate the effects of recombinant human interleukin-1α（rhIL-1α） on the expression of receptor activator of NF-κB ligand（RANKL） and osteoprogeterin（OPG） in human periodontal ligament fibroblasts（HPDLFs）.Methods： HPDLFs were isolated and cultured in media containing different concentrations of rhIL-1α（0、5、10 or 20μg/L）.After 24h incubation,fluorescent quantitative RT-PCR（FQ-RT-PCR） was performed to detect the expression of RANKL and OPG mRNA using GAPDH mRNA as internal control.Results： rhIL-1α significantly up-regulated the mRNA expression of RANKL and OPG.Furthermore,the RANKL/OPG ratio was increased after stimulated by rhIL-1α.When the concentration was 10μg/L,the effect was most obvious.Conclusion： RhIL-1α had effects on the expression of RANKL and OPG.It regulated the RANKL/OPG ratio in HPDLFs.RhIL-1α may play an important role in the alveolar bone remodeling.

关 键 词：核因子kB受体活化因子配基 骨保护素 人牙周膜成纤维细胞 重组人白介素-1α 

分 类 号：R78[医药卫生—口腔医学]