二烯丙三硫抑制人前列腺癌细胞生长  

Mechanism of Growth of Human Prostate Cancer Cells Inhibited by DATS

作　　者：郝建伟[1] 丘少鹏[1] 毛晓鹏[1,2] 陈羽[1] 郭胜杰[1] 黄斌[1] 

机构地区：[1]中山大学附属第一医院泌尿外科,广东广州510080 [2]中山大学附属第一医院东区外科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2010年第2期186-189,194,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30872584);教育部博士点基金(20050558065);广东省自然科学基金博士启动项目(9451008901002062);广东省自然科学基金重点项目(8251008901000018)

摘　　要：【目的】研究二烯丙三硫(DATS)对人前列腺癌PC-3细胞的生长抑制作用及相关机制。【方法】MTT法测定DATS对PC-3细胞生长的影响;细胞形态学,流式细胞仪(FCM)等检测细胞凋亡;western blot、比色法分别检测凋亡相关蛋白Bcl-2,Bax和Bcl-xL/Bcl-xS的表达以及Caspase-3活性变化。【结果】DATS明显抑制PC-3细胞的生长,呈浓度和时间依赖性;DATS作用72h的IC50为14μmol/L;14μmol/LDATS作用PC-3细胞不同时间后,细胞凋亡率增高;western blot显示凋亡抑制蛋白Bc1-2、Bcl-xL表达减少,比色法表明Caspase-3活性增高。【结论】DATS诱导细胞凋亡是其抑制前列腺癌PC-3细胞生长的重要机制,该机制可能与Bc1-2、Bcl-xL和Caspase-3表达及活性变化有关。【Objective】 This study was designed to determine growth inhibition of diallyl trisulfide （DATS） in human prostate cancer cells by inducing apoptosis and further to investigate the mechanism underlying such effect. 【Methods】 Growth inhibition by DATS was estimated by the tetrazolium （MTT） assay. Apoptosis induction in DATS-treated cells was assessed by fluorescence microscopy analysis of cells with condensed and segmented nuclei following staining with DAPI and flow cytometric analysis of cells with sub-G1 DNA content following staining with propidium iodide. Protein levels of apoptosis regulating proteins were determined using western blot. The activity of caspase-3 was measured using a colorimetric assay. 【Result】 DATS showed tumor growth inhibition in a time- and dose-dependent manner, IC50 of DATS was 14 μmol / L at 72 h. DATS evoked apoptosis as confirmed by cell morphology and by the analysis of flow cytometry. The expression of Bcl-2 and Bcl-xL, the apoptosis-suppressing proteins, was more down-regulated. The activity of caspase-3 was enhanced by DATS. 【Conclusion】 DATS inhibits growth of prostate cancer cells by inducing apoptosis in association with down-regulation of Bcl-2 and Bcl-xL and activation of caspase-3.

关 键 词：二烯丙三硫 凋亡 前列腺癌 

分 类 号：R737.25[医药卫生—肿瘤]