N-甲基-N-顺式-苯乙烯基-桂皮酰胺对青霉素致痫大鼠痫样放电的影响  

Effects of N-methyl-N-cis-styryl-cinnamamide on Penicillin-induced Epileptiform Discharges in Rats

作　　者：邬继栋[1] 何斯纯[2] 黄雪松[3] 沈伟哉[4] 

机构地区：[1]暨南大学医学院医学院人体解剖学教研室,广东广州510632 [2]暨南大学医学院医学院生理学教研室,广东广州510632 [3]暨南大学医学院食品科学与工程系,广东广州510632 [4]暨南大学医学院人体解剖学教研室,广东广州510632

出　　处：《中山大学学报（医学科学版）》2010年第2期213-220,共8页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30471216);广东省自然科学基金团队项目(039213)

摘　　要：【目的】探讨黄皮核提取物N-甲基-N-顺式-苯乙烯基-桂皮酰胺对青霉素(PNC)诱发大鼠癫痫的影响。【方法】32只SD大鼠随机分成正常对照组(20mL/L吐温80+生理盐水)、模型对照组(20mL/L吐温80+PNC)、低剂量桂皮酰胺组(桂皮酰胺75mg/kg+PNC)、高剂量桂皮酰胺组(桂皮酰胺150mg/kg+PNC),每组8只。建立大鼠皮层定位注射青霉素诱发惊厥模型,观察并分析两剂量的桂皮酰胺腹腔注射前处理给药对造模后大鼠惊厥发作程度和皮层脑电图的影响。【结果】两种剂量的N-甲基-N-顺式-苯乙烯基-桂皮酰胺均可减轻大鼠癫痫发作程度,延长痫样放电的潜伏期,减少痫波持续时间,在发作后期减少痫波发放频率,与模型对照组比较,差异明显(P<0.05或P<0.01)。然而,对于棘波最高和最低波幅的影响,桂皮酰胺组与模型对照组比较差异无统计学意义(P>0.05)。【结论】天然产物黄皮核提取物N-甲基-N-顺式-苯乙烯基-桂皮酰胺对大鼠皮层定位注射青霉素诱发的惊厥发作和痫样放电具有明显的抑制作用,该天然产物具有一定的抗惊活性。【Objective】 To investigate the effects of the seed extract of Clausena lansium, N-methyl-N-cis-styryl-cinnamamide, on the penicillin-induced epilepsy in rats. 【Methods】 Thirty-two Sprague-Dawely rats were randomly divided into four groups with 8 rats in each group： normal control （20 mL/L Tween 80 ＋ Normal Saline）, model control （20 mL/L Tween 80 ＋ Penicillin）, low dose treatment （Cinnamamide 75 mg/kg ＋ Penicillin）, high dose treatment （Cinnamamide 150 mg/kg ＋ Penicillin）. The epilepsy rat model was established by localized intracortical injections of penicillin. The effects of pretreatments of the rats with N-methyl-N-cis-styryl-cinnamamide （intraperitoneal injection, ip） on the penicillin-induced seizure activities and the epileptiform discharges recorded by electrocorticogram （ECoG） were observed. 【Results】 The penicillin-induced seizure was significantly lightened in both the pretreatment groups （75 and 150 mg/kg N-methyl-N-cis-styryl-cinnamamide）, compared with the model group. N-methyl-N-cis-styryl-cinnamamide （75 and 150 mg/kg） could significantly prolonged the latency of epileptiform activities, shorten the duration of epileptiform discharges and decreased the mean spike frequency in the late stage （P〈0.05 or P〈0.01）. However, there was no significant difference in the highest value or the lowest value of the epileptiform waves between the model and cinnamamide treatment groups. 【Conclusion】 The seed extract of Clausena lansium, N-methyl-N-cis-styryl-cinnamamide, can significantly inhibit the seizure and epileptiform discharges caused by penicillin-localized injections in the cortex of rats, suggesting that the natural substance has an anticonvulsive function.

关 键 词：黄皮核 N-甲基-N-顺式-苯乙烯基-桂皮酰胺 癫痫 青霉素诱发 痫样放电 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R284.2[医药卫生—临床医学]