肿瘤浸润树突状细胞和T淋巴细胞在EBV相关胃癌中的肿瘤免疫作用  

Roles of Tumor Infiltrating Dendritic Cells and T Lymphocytes in Tumor Immunity of EBV-associated Gastric Carcinoma

作　　者：肖琳[1] 何丹[1] 丁运刚[2,3] 冯智英[1] 邵春奎[1] 

机构地区：[1]中山大学附属第三医院病理科,广东广州510630 [2]中山大学中山眼科中心 [3]国家眼科重点实验室,广东广州510060

出　　处：《中山大学学报（医学科学版）》2010年第2期253-257,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30672409);广东省自然科学基金(8151008901000132;05001748)

摘　　要：【目的】探讨肿瘤浸润树突状细胞(TIDC)及肿瘤浸润T淋巴细胞(TIL-T)在EBV相关胃癌(EBVaGC)中的肿瘤免疫作用及其临床意义。【方法】采用免疫组织化学方法检测49例EBVaGC和20例EBV阴性胃癌(EBVnGC)中TIDC及TIL-T的浸润程度及亚型,并分析其与EBVaGC的临床病理特征的关系。【结果】TIDC在49例EBVaGC中有29例(59.2%)呈轻度浸润,20例(40.8%)为显著浸润,而EBVnGC中则有18例(90%)为轻度浸润,2例(10%)为显著浸润。经统计学分析,TIDC在EBVaGC显著浸润率高于EBVnGC(P=0.013)。TIL-T在EBVaGC的数量较EBVnGC多(P=0.017),且多数为CD8+细胞毒性T细胞(CTL);EBVaGC中TIL-T与TIDC数量呈正相关(r=0.386,n=49,P=0.006)。TIL-T浸润程度与淋巴结转移呈负相关(P<0.001),而TIDC浸润程度与临床病理特征无关。【结论】TIDC及TIL-T可能在EBVaGC肿瘤免疫反应中起着重要的作用,TIDC可能对于TIL-T具有趋化作用。【Objective】 To explore the roles of TIDC （tumor infiltrating dendritic cell） and TIL-T （tumor infiltrating T lymphocyte） in tumor immunity of EBV-associated gastric carcinoma （EBVaGC） and their clinical significance. 【Methods】 TIDC and TIL-T in EBVaGC （n = 49） and EBVnGC （EBV-negative gastric carcinoma） （n=20） were detected by immunohistochemistry method and then their subtypes and their relationships with clinical and pathological factors were analyzed. 【Results】 Mild infiltration was detected in 29 of 49 （59.2%） EBVaGCs and 18 of 20 （90%） EBVnGCs, while marked infiltration of TIDC was detected in 20 of 49 （40.8%） EBVaGCs and 2 of 20 （10%） EBVnGCs. And the difference between the two groups was significant （P=0.013）. The number of TIL-T in EBVaGC was higher than that in EBVnGC （P=0.017）, and most of them were CTLs. The number of TIL-T was positively correlated with that of TIDC （r = 0.386, n = 49, P = 0.006）. The degree of TIL-T infiltration in EBVaGC had negative relationship with the lymph node metastasis （P〈 0.001）, while TIDC had no relationship with the clinical features. 【Conclusion】 TIDC and TIL-T may play important roles in the tumor immunity of EBVaGC; TIDC may contribute to the TIL-T recruitment in EBVaGC.

关 键 词：EB病毒相关胃癌 肿瘤浸润性T淋巴细胞 肿瘤浸润性树突细胞 

分 类 号：R735.2[医药卫生—肿瘤]