新城鸡瘟病毒激活巨噬细胞释放一氧化氮及对肝癌瘤株细胞毒活性的研究  

The experimental study of the cytotoxicity of murine peritoneal elicit macrophage infected by newcastle disease virus strain

作　　者：白莲花[1] 刘倩[1] 李娟[1] 陈乃玲[1] 

机构地区：[1]北京军区总医院全军肝病研究所

出　　处：《军医进修学院学报》1999年第1期40-42,共3页Academic Journal of Pla Postgraduate Medical School

基　　金："九五"军队医药卫生科研基金

摘　　要：目的：分析新城鸡瘟病毒Ｌｏｓｓｔａ系（ＮＤＶ－Ｌ）对小鼠腹腔巨噬细胞是否具有激活作用并对其机制进行探讨。方法：采用电镜、氚标记的胸腺嘧啶核苷（３Ｈ－ＴｄＲ）和Ｇｒｉｅｓｓ方法。结果：①ＮＤＶ－Ｌ与小鼠腹腔巨噬细胞（ＰＥＭ）作用２４ｈ后，光镜和扫描镜均显示细胞明显增大和伪足伸出；②被ＮＤＶ－Ｌ作用的ＰＥＭф与３Ｈ－标记的Ｈ２２靶细胞作用２４ｈ后，发现具有明显的细胞毒杀伤效应，且该效应与阳性对照组〔卡介苗、细菌内毒素联合激活的ＰＥＭф（ＢＣＧ－ＬＰＳ－ＰＥＭф）〕的杀伤效应接近；③收集ＮＤＶ－Ｌ与ＰＥＭф作用２４ｈ上清进行检测，发现一氧化氮（ＮＯ）分泌量增高。Objective:The cytotoxicity of murine peritoneal elicit macrophage(PEMф) infected by NDV L was studied in vitro. Methods:This study was carried out by electronic microscope, 3H TdR and Griess assay. Results:(1)Under the optical microscope and scan electronic microscope, the shape of the PEMф changed markedly comparing with the control group, while the NDV L infected PEMф for 24 hours; (2)When the activated PEMф infected by NDV L acted on H 22 and P 815 tumor cells, a significant tumorcidal effect was found and the cytotoxicity was nearly the same as that in the positive group (BCG LPS PEMф); (3)while infecting murine PEMф for 24 hours, NDV L could induce the PEMф releasing nitric oxide (NO). Conclusion:The PEMф activated by NDV L may kill H 22 and P 815 tumor cells and this function seems to be related with NO releasing.

关 键 词：新城鸡瘟病毒 巨噬细胞 一氧化氮 肝癌 

分 类 号：R735.705[医药卫生—肿瘤]