大麻受体激动剂WIN-55,212-2对肝癌细胞HepG2增殖和凋亡的影响  

The effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells

作　　者：王映[1] 周于婷[2] 赵青[1] 

机构地区：[1]广州医学院生化教研室,广东广州510182 [2]南方医科大学生物技术学院,广东广州510515

出　　处：《细胞与分子免疫学杂志》2010年第4期344-347,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：教育部留学回国人员科研启动基金资助项目(教外司留2007-1108号);广州市教育局市属高校科技计划项目(61057;08A112);广州市科技计划项目(2009J1-C361);广东省中医药局建设中医药强省科研课题(2008362);广州市留学回国人员科研启动基金(2007-583);广州医学院留学回国人员启动科研基金(2006GD052)

摘　　要：目的:探讨激活大麻受体对肝癌细胞增殖和凋亡的作用,并对其机制进行初步探讨。方法:将细胞分成对照组、不同剂量大麻受体激动剂WIN-55,212-2(WIN)处理组。MTT法测定WIN对HepG2细胞增殖的影响;DNA梯度电泳法检测HepG2细胞凋亡DNA片段,流式细胞术检测分析各组细胞凋亡率变化,Western blot分析各组细胞caspase-3和c-myc的蛋白表达,分光光度法检测caspase-3、8、9的活性。结果:大麻受体激动剂WIN能抑制肝癌细胞生长和c-myc的蛋白表达,诱导肝癌细胞凋亡,上述效应具有剂量依赖性。而且WIN可能通过诱导细胞caspase-3的蛋白表达和激活caspase-3、8、9活性进而诱导肝癌细胞凋亡。结论:大麻受体激动剂WIN能抑制肝癌细胞生长,诱导肝癌细胞凋亡,并与caspases和c-myc的蛋白表达相关。AIM：To study the effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells. METHODS：HepG2 cells were treated with different doses of the activator of cannabinoid receptor-WIN-55,212-2（WIN）,MTT was used to detect the effects of WIN on HepG2 cells; Flow cytometry （FCM） was used to examine the effects of WIN on the apop tosis of HepG2 cells. Caspase 3 and c-myc proteins were determined by Western blot assay,and caspase 3,8,9 activity was measured using a colorimetric method. RESULTS：WIN could inhibit HepG2 cell proliferation and induce cell apoptosis dose-dependently. WIN increased caspase 3 protein levels and caspase 3,8,9 activity. CONCLUSION：WIN can induce apoptosis,the effect might be associated with up-regulating expression of caspases protein and down-regulating expression of c-myc protein.

关 键 词：WIN-55 212-2 肝癌细胞HEPG2 凋亡 增殖 

分 类 号：R73-361[医药卫生—肿瘤]