大黄素对作用实验性牙周炎模型及NO的影响  

Therapeutic effect of emodin in experimental periodonititis rats

作　　者：刘冰[1] 李淑娟[1] 杨冬茹[1] 董福生[1] 黄香河[1] 李涛[1] 

机构地区：[1]河北医科大学口腔医院口腔内科,河北石家庄050017

出　　处：《细胞与分子免疫学杂志》2010年第4期391-393,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：河北省科学技术研究与发展计划项目(07276457);河北省卫生厅中医;中西医结合科研计划项目(2006055)

摘　　要：目的:通过检测实验性牙周炎大鼠牙龈组织及外周血中NO的变化,探讨大黄素治疗牙周炎的机制。方法:选取纯种雌性SD大鼠随机分为4组,各30只;正常对照组(N组)、牙周炎组(P组)、低浓度大黄素治疗组(PL组)及高浓度大黄素治疗组(PH组)。建立牙周炎动物模型并按分组用药,分别于4、6、8周采用股动脉放血法处死大鼠,留取血清,检测血清中一氧化氮(NO)水平。同时取一侧上颌骨做光镜形态学观察,取另一侧上颌骨结扎牙周围的牙龈组织测量NO含量,并作统计学分析。结果:PL和PH组大鼠的牙龈组织及外周血中的NO在实验的各阶段均明显低于P组(P<0.05)。结论:大黄素可以通过降低牙周炎大鼠牙龈及外周血中NO的含量,对牙周炎有一定的治疗意义。AIM：Throught observing the change of Nitric Oxide （NO） in the peripheral blood and gingival tissue,to explore the mechanism of Emodin （EMD） in the therapy of periodontal disease. METHODS：SD rats were randomly divided into four groups of thirty （group N,group P,group PL and the group PH）. The periodontitis model was made and EMD was administered at PL and PH. At the 4th,6th,8th weeks after the animal models were established,the rats were killed respectively and the maxilla and the peripheral blood were collected. A side of maxilla was stained with HE for histological examination and the other side of gingival tissue surround ligated tooth was collected for the measurement of NO. Also,it was measured in the peripheral blood. GLM method using SPSS was used to compare changes in each of the variables over time. RESULTS：The levels of NO in the peripheral blood and gingival tissue in PL and PH groups were significantly lower than that in P groups（P〈0.05）. CONCLUSION：Emodin can take effect on periodontitis by reducing the levels of NO in the peripheral blood and gingival tissue.

关 键 词：大黄素 NO 牙周炎 牙龈组织 外周血 

分 类 号：R781.43[医药卫生—口腔医学]