黑素瘤细胞中脂肪酸结合蛋白7的表达及其与细胞增殖、凋亡的关系  

Expression of Fatty Acid Binding Protein 7 in Melanoma and Its Relationship with Proliferation and Apoptosis of Melanoma Cells

作　　者：钟桂书[1] 任培蓉[2] 黄忠奎[3] 史丙俊[4] 熊霞[1] 

机构地区：[1]泸州医学院附属医院皮肤科,四川泸州646000 [2]泸州医学院附属医院肿瘤科,四川泸州646000 [3]绵阳市中心医院皮肤科,四川绵阳621000 [4]杭州师范大学附属医院皮肤科,浙江杭州310035

出　　处：《中国皮肤性病学杂志》2010年第5期398-400,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的探讨黑素瘤细胞中脂肪酸结合蛋白7(FABP7)的表达及其与细胞增殖、凋亡的关系。方法采用免疫组织化学方法分析临床45例良性痣(为皮内痣)、60例原位黑素瘤、60例转移性黑素瘤标本中FABP7的表达。取组织细胞进行体外培养,选择FABP7特异性小干扰RNA(siRNA)降低FABP7的表达,然后检测黑素瘤细胞的增殖及凋亡情况。结果良性痣细胞浆及细胞核中FABP7的表达均高于原位黑素瘤、转移性黑素瘤(P<0.05),原位黑素瘤和转移性黑素瘤细胞浆及细胞核中的FABP7表达差异无显著性(P>0.05)。下调FABP7表达可减少原位黑素瘤细胞31.0%及转移性黑素瘤细胞81.0%的增殖性,下调FABP7表达对原位黑素瘤及转移性黑素瘤细胞的凋亡率无明显影响。结论良性痣中FABP7表达比黑素瘤高,FABP7对体外培养黑素瘤细胞增殖有影响。Objective To investigate the expression of fatty acid binding protein 7 （FABP7） in melanoma and its relationship with proliferation and apoptosis of melanoma cells. Methods Clinical samples from 45 cases of benign nevi, 60 cases of primary melanoma, and 60 cases of metastatic melanoma were analyzed by immuno-histochemistry. Melanoma cells were cultured in vitro and treated with specific FABP7 small interfering RNA to reduce the expression of FABP7, then the proliferation and apoptosis of these cells were calculated. Resuits The cytoplasmic and nuclear expression of FABP7 in nevi was significantly higher than those in primary or metastatic melanoma （ P 〈 0.05 ）, and there was no significant difference between the later two groups （P 〉 0.05 ）. The down-regulation of FABP7 led to 31.0% reduction of proliferation of primary melanoma cells and 81.0% reduction of proliferation of metastatic melanoma cells, but had no significant effects on apoptosis. Conclusion FABP7, which is higher expressed in nevi than in melanoma, can regulate the proliferation of melanoma cells in vitro.

关 键 词：黑素瘤 脂肪酸结合蛋白质类 细胞增殖 细胞凋亡 

分 类 号：R739.5[医药卫生—肿瘤]