化疗药对人肝癌细胞SMMC-7721端粒酶活性的影响  

Inhibition of telomerase activity of human liver cancer cell SMMC 7721 by chemotherapeutic drugs

作　　者：孟志强[1] 于尔辛[1] 宋明志[1] 

机构地区：[1]上海医科大学肿瘤医院肝癌科

出　　处：《世界华人消化杂志》1999年第3期252-254,共3页World Chinese Journal of Digestology

摘　　要：目的研究常用化疗药物对人肝癌细胞ＳＭＭＣ７７２１端粒酶活性的影响．方法利用端粒重复扩增实验（ＴＲＡＰ）结合非变性聚丙烯酰胺凝胶电泳银染法，测定ＳＭＭＣ７７２１细胞经过几种化疗药物（顺铂、阿霉素、丝裂霉素、５ＦＵ）不同浓度和时间作用后端粒酶活性的变化．结果当各种药物大剂量处理细胞４ｈ后再祛除药物培养２０ｈ后，顺铂对酶的活性完全抑制，丝裂霉素有部分抑制，阿霉素和５ＦＵ无抑制作用；而未经２０ｈ再培养则无作用；小剂量药物处理细胞５ｄ，其结果同大剂量相似．结论顺铂和丝裂霉素对人肝癌ＳＭＭＣ７７２１细胞端粒酶活性有抑制作用，可能与药物的作用机制有关．AIM To investigate the inhibition of telomerase activity by several chemotherapeutic drugs regularly used in liver cancer treatment. METHODS By using telomerase repeat amplification protocol (TRAP) combined with PAGE silver staining, we detected the changing of telomerase activity of human liver cancer cell line SMMC 7721 by cisplatin, 5 FU, doxorubicin, mitomycin C in different doses and different periods. RESULTS In high doses, no inhibition of tolemerase activity was found when cells were collected after only 4 hours of drug treatment, but the telomerase activity of cells was completely inhibited by cisplatin and partly inhibited by mitomycin C when the drug was removed and cells recultured for 20 hours. However, doxorubicin and 5 FU had no effect.In low doses,the effects were similar. CONCLUSION Cisplatin and mitomycin C can inhibit telomerase activity of human liver cancer SMMC 7721 cell, which maybe correlates with their anti cancer mechanism.

关 键 词：肝肿瘤 药物疗法 端粒酶 顺铂 丝裂霉素C 

分 类 号：R735.705[医药卫生—肿瘤] R730.53[医药卫生—临床医学]