κ-卡拉胶固相酸降解及其寡糖结构分析  

Solid Acid Hydrolysis of κ-carrageenan and the Structural Analyses of Oligosaccharides

作　　者：郝翠[1] 于广利[1] 赵峡[1] 杨波[1] 李广生[1] 郇培[1] 郝杰杰[1] 曾洋洋[1] 

机构地区：[1]中国海洋大学医药学院糖科学与糖工程实验室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2010年第5期19-22,共4页Periodical of Ocean University of China

基　　金：科技部国际合作项目(2007DFA30980);国家海洋高技术研究发展计划项目(2007AA09Z445)资助

摘　　要：以κ-卡拉胶为原料,采用正交实验法建立了1种固相酸降解制备κ-卡拉胶寡糖方法。结果表明,当阳离子交换树脂(732#)用量为100 mg/mL、κ-卡拉胶的质量浓度为3%时,在60℃下降解3 h后,寡糖分布宽度较窄,经PAGE和电喷雾离子化质谱(ESI-MS)分析,表明所得寡糖为聚合度小于25的系列奇数寡糖。In this Paper,the solid acid hydrolysis method was established to prepare κ-carrageenan oligosaccharides by the orthogonal experiment.The results showed that the oligosaccharide distribution width was narrow under the following condition of 100 mg/mL 732^# resin,3% κ-carrageenan,60 ℃,reacting for 3 hours.The degree of polymerization was under 25 by PAGE analysis,and their structure was confirmed to be series of odd-numbered κ-carrigeenan oligosaccharides by ESI-MS.

关 键 词：固相酸降解 Κ-卡拉胶 奇数寡糖 结构 

分 类 号：Q949.293.30[生物学—植物学] R282.77[医药卫生—中药学]