上海地区弥漫性大B细胞淋巴瘤的生发中心B细胞样型显著偏低  

Prevalence of germinal center B-cell-like and non-germinal center B-cell-like types of diffuse large B-cell lymphoma in Shanghai, China

作　　者：陈燕[1] 陈慧[2] 付凯 朱雄增[1] Richard Irons 

机构地区：[1]复旦大学附属肿瘤医院病理科复旦大学上海医学院肿瘤学系,200032 [2]复旦大学中美联合分子医学研究中心 [3]Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, U S A

出　　处：《中华病理学杂志》2010年第5期313-318,共6页Chinese Journal of Pathology

摘　　要：目的探讨中国上海地区弥漫性大B细胞淋巴瘤（DLBCL）的生发中心B细胞（GCB）样型与非GCB样型的分布以及可能影响因素。方法应用免疫组织化学EnVision法分析124例来自该院的原发DLBCL中CD10、bcl-6、MUM1、GCET1和FOXP15种蛋白的表达，采用Hans和Choi两种免疫表型分型法进行分型。其中118例应用荧光原位杂交技术检测t（14；18）和bcl-6基因重排情况。结果使用Hans法对124例DLBCI．进行分型，27例（22％）为GCB样型，97例（78％）为非GCB样型。使用Choi法进行分型显示34例（27％）为GCB样型，90例（73％）为非GCB样型（即ABC样型）。GCB样型显著低于非GCB样型（P=0．0001）。t（14；18）易位仅4例（3％），3例发生于GCB样型中。bcl-6基因重排阳性的病例为46例（39％），高发于GCB样型中。bcl-6基因重排与bcl-6蛋白表达没有明显相关性。结论中国上海地区DLBCL的GCB样型显著低于非GCB样型，可能与其DLBCL的t（14；18）低水平发生有关。Objective To study the prevalence of germinal center B-cell-like （GCB） and non-GCB-like types of diffuse large B-cell lymphoma （DLBCL） in Chinese patients. Methods Immunohistochemical study for CD10, bcl-6, MUMI, GCET1 and FOXP1 was performed on 124 cases of DLBCL from Shanghai, China. The Hans algorithm and Choi algorithm were applied to classify DLBCL into GCB and non-GCB-like types. Fluorescence in-situ hybridization （FISH） for t （14; 18） and bcl-6 gene rearrangement was also carried out on 118 cases. Results Of the 124 DLBCL cases studied, 27 cases （22%） showed a GCB-like type and 97 cases （78%） showed a non-GCB-like type, when using Hans algorithm. On the other hand, 34 cases （27%） belonged to GCB-like type and 90 cases （73%） belonged to non-GCB-like type when applying Choi algorithm. The prevalence of GCB-like type was significantly lower than that of non-GCB-like type （ P = 0. 0001 ）. Only four cases （3%） were positive for t （ 14 ; 18 ）, and three of them were classified as GCB-llke type. bcl-6 rearrangement was found in 46 cases （39%） and more frequently encountered in the GCB-like type. There is no relationship between bcl-6 gene rearrangement and bcl-6 protein expression. Conclusions The GCB-like type of DLBCL is significantly less common than non-GCB-like type in Chinese population. This phenomenon is possibly related to the low frequency of t（14;18）.

关 键 词：淋巴瘤 B细胞 免疫表型分型 易位 遗传 原癌基因蛋白质类c—bcl-6 

分 类 号：R733.1[医药卫生—肿瘤]