卵巢癌紫杉醇耐药的蛋白质组学研究(英文)  

Proteomic analysis of human ovarian cancer paclitaxel-resistant cell lines

作　　者：曹兰琴[1] 黎欣[1] 张怡[1] 彭芳[2] 易红[2] 许艳[2] 李新国[1] 王前[1] 

机构地区：[1]中南大学湘雅医院妇产科,长沙410008 [2]中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙410008

出　　处：《中南大学学报（医学版）》2010年第4期286-294,共9页Journal of Central South University ：Medical Science

基　　金：supported by the Department of Science and Technology of Hunan Province, P.R.China(08RS4018)

摘　　要：目的:研究与卵巢癌紫杉醇耐药相关的蛋白质。方法:应用双向凝胶电泳( 2-DE)和基质辅助激光解吸电离飞行时间质谱( MALDI-TOF-MS)寻找紫杉醇耐药细胞和敏感细胞的差异表达蛋白质。使用Western印迹技术对其中2个蛋白质进行验证。结果:通过对2组细胞总蛋白质双向凝胶电脉图谱进行分析,找到差异蛋白质点40个;通过质谱分析,24个蛋白质得到鉴定。这些蛋白质包括增殖细胞核抗原( PCNA)、nm23蛋白、prohibitin( PHB)分子伴侣蛋白、脂皮质素( annexin)、α-烯醇化酶(α-enolase)以及热休克蛋白( HSP)等。结论:通过蛋白质组学技术,发现了卵巢癌紫杉醇耐药细胞系和敏感细胞系之间差异表达蛋白质24个,这些差异蛋白质可能参与卵巢癌细胞紫杉醇耐药过程。Objective To study the proteins related to paclitaxel-resistant of ovarian cancer cell line.Methods The total proteins of paclitaxel-resistant and paclitaxel-sensitive human ovarian cancer cell lines were separated by 2-dimensional gel electrophoresis （2-DE）.The differentially expressed proteins were analyzed using image analysis software.The differential proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Western blot was used to determine the differential expression levels of the 2 proteins.Results Forty differentially expressed proteins were found by image analysis software,and 24 differential proteins were identified by mass spectrometry.These proteins included proliferation cell nuclear antigen （PCNA）,nm23,prohibitin （PHB）,annexin,α-enolase,heat shock protein （HSP）,and so on.Conclusion Twenty-four proteins in human ovarian cancer cell lines of paclitaxel-resistant and paclitaxel-sensitive are found by proteomic techniques,which may be involved in the paclitaxel-resistance of human ovarian cancer cells.

关 键 词：卵巢癌 紫杉醇 耐药 蛋白质组学 

分 类 号：R737.31[医药卫生—肿瘤]